Analysis of DNA topology of EBV minichromosomes in HEK 293 cells
Correspondencia: schvartzman@cib.csic.es (JBS); mjfernes@hotmail.com (JFN).
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , |
| Format: | article |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | https://doi.org/10.1371/journal.pone.0188172 http://hdl.handle.net/20.500.14066/3345 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1870612065213743104 |
|---|---|
| author | Castán, Alicia |
| author2 | Fernández Calleja, Vanessa Hernández, Pablo Krimer, Dora B. Schvartzman, Jorge Bernardo Fernández de Nestosa, María José |
| author2_role | author author author author author |
| author_browse | Castán, Alicia Fernández Calleja, Vanessa Fernández de Nestosa, María José Hernández, Pablo Krimer, Dora B. Schvartzman, Jorge Bernardo |
| author_facet | Castán, Alicia Fernández Calleja, Vanessa Hernández, Pablo Krimer, Dora B. Schvartzman, Jorge Bernardo Fernández de Nestosa, María José |
| author_role | author |
| bitstream.checksum.fl_str_mv | 06772a6a174ad38d400ef1494584db54 858b22fda432bd774e469302988c1974 b8e439a7c4803fb884aee6c68a5694e6 b8e439a7c4803fb884aee6c68a5694e6 2f25a9f553e95879bc940ade56f9b08c |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 MD5 MD5 MD5 |
| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/1/Analysis%20of%20DNA%20topology%20of%20EBV%20minichromosomesinHEK293cells.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/2/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/3/14-INV-062art1.pdf.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/5/Analysis%20of%20DNA%20topology%20of%20EBV%20minichromosomesinHEK293cells.pdf.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/4/Analysis%20of%20DNA%20topology%20of%20EBV%20minichromosomes%20in%20HEK%20293%20cells.jpg |
| dc.contributor.other.es.fl_str_mv | Universidad Nacional de Asunción. Facultad Politécnica |
| dc.creator.none.fl_str_mv | Castán, Alicia Fernández Calleja, Vanessa Hernández, Pablo Krimer, Dora B. Schvartzman, Jorge Bernardo Fernández de Nestosa, María José |
| dc.date.accessioned.none.fl_str_mv | 2022-04-24T14:59:57Z |
| dc.date.available.none.fl_str_mv | 2022-04-24T14:59:57Z |
| dc.date.issued.none.fl_str_mv | 2017-11-29 |
| dc.format.extent.es.fl_str_mv | 16 páginas |
| dc.identifier.citation.en.fl_str_mv | Castán, A., Fernández Calleja, V., Hernández, P., Krimer, D. B., Schvartzman, J. B., & Fernández Nestosa, M. J. (2017). Analysis of DNA topology of EBV minichromosomes in HEK 293 cells. PLoS ONE, 12(11), Artículo e0188172. https://doi.org/10.1371/journal.pone.0188172 |
| dc.identifier.doi.es.fl_str_mv | 10.1371/journal.pone.0188172 |
| dc.identifier.essn.es.fl_str_mv | 1932-6203 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.1371/journal.pone.0188172 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/3345 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.en.fl_str_mv | Public Library of Science |
| dc.relation.projectCONACYT.es.fl_str_mv | 14-INV-062 |
| dc.rights.*.fl_str_mv | Attribution 4.0 International |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.classification.en.fl_str_mv | 6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations |
| dc.subject.classification.es.fl_str_mv | 6. Producción y tecnología industrial |
| dc.subject.other.es.fl_str_mv | Antígenos nucleares del virus de Epstein-Barr/genética Células HEK293 Genes virales Herpesvirus humano 4 Humanos Epstein-Barr virus nuclear antigens/genetics HEK293 Cells Genes, viral Herpesvirus 4, Human/genetics Humans |
| dc.title.es.fl_str_mv | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Correspondencia: schvartzman@cib.csic.es (JBS); mjfernes@hotmail.com (JFN). |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_126bb84d9b0a9f53f6fde8a39afe530e |
| identifier_str_mv | Castán, A., Fernández Calleja, V., Hernández, P., Krimer, D. B., Schvartzman, J. B., & Fernández Nestosa, M. J. (2017). Analysis of DNA topology of EBV minichromosomes in HEK 293 cells. PLoS ONE, 12(11), Artículo e0188172. https://doi.org/10.1371/journal.pone.0188172 10.1371/journal.pone.0188172 1932-6203 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/3345 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| spelling | c33ad711-0e80-408a-a604-c155d584db17600a355d0b0-23b4-480f-9273-6490411036b9600644c6599-893c-4718-9a69-f27695fa83916001a615720-7aa0-4b4c-b423-547a688589cf6001065600411600Universidad Nacional de Asunción. Facultad Politécnica2022-04-24T14:59:57Z2022-04-24T14:59:57Z2017-11-29Castán, A., Fernández Calleja, V., Hernández, P., Krimer, D. B., Schvartzman, J. B., & Fernández Nestosa, M. J. (2017). Analysis of DNA topology of EBV minichromosomes in HEK 293 cells. PLoS ONE, 12(11), Artículo e0188172. https://doi.org/10.1371/journal.pone.0188172https://doi.org/10.1371/journal.pone.0188172http://hdl.handle.net/20.500.14066/334510.1371/journal.pone.01881721932-6203Correspondencia: schvartzman@cib.csic.es (JBS); mjfernes@hotmail.com (JFN).Simian Virus 40 (SV40) andEpstein-Barr Virus (EBV) are frequently used as model systems to study DNAreplication. Their genomes are both circular duplex DNAs organized in a single replicon where replication initiates at a precise site upon binding of a specific protein: the large tumor (T) antigen for SV40 and the Epstein-Barr Nuclear Antigen 1 (EBNA-1) for EBV. Despite the abundant information available on the genetics and biochemistry of the replication process in these systems, little is known about the changes in DNA topology that take place as molecules are transfected into eukaryotic cells, assembled into chromatin and bind initiator proteins to start replication. Here we used high-resolution two-dimensional agarose gel electrophoresis to demonstrate that in Human Embryonic Kidney (HEK) 293 cells, minichromosomes ofalmost the samemasscarrying either the SV40 or the EBV replication origin showed similar topological features. The patterns were very similar regardless of the initiator proteins. We also showed that in a hybrid minichromosome, pEco3'Δ, that initiates replication from the SV40 origin, the presence of EBNA-1 and its putative binding to the EBV ªfamily of repeatsº induces no significant topological change. These observations challenge the idea that binding of EBNA-1 to oriP could induce negative supercoiling and favor a model suggesting that it binds to oriP in a two-step process where only the second step causes structural changes in a transient cell cycle specific manner.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrollo16 páginasengPublic Library of ScienceAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2017 Castan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.6. Producción y tecnología industrial6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparationsAntígenos nucleares del virus de Epstein-Barr/genéticaCélulas HEK293Genes viralesHerpesvirus humano 4HumanosEpstein-Barr virus nuclear antigens/geneticsHEK293 CellsGenes, viralHerpesvirus 4, Human/geneticsHumansAnalysis of DNA topology of EBV minichromosomes in HEK 293 cellsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11PLoS ONE14-INV-06212Castán, AliciaFernández Calleja, VanessaHernández, PabloKrimer, Dora B.Schvartzman, Jorge BernardoFernández de Nestosa, María JoséORIGINALAnalysis of DNA topology of EBV minichromosomesinHEK293cells.pdfAnalysis of DNA topology of EBV minichromosomesinHEK293cells.pdfArtículo científicoapplication/pdf4816860http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/1/Analysis%20of%20DNA%20topology%20of%20EBV%20minichromosomesinHEK293cells.pdf06772a6a174ad38d400ef1494584db54MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/2/license.txt858b22fda432bd774e469302988c1974MD52TEXT14-INV-062art1.pdf.txt14-INV-062art1.pdf.txtExtracted texttext/plain48049http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/3/14-INV-062art1.pdf.txtb8e439a7c4803fb884aee6c68a5694e6MD53Analysis of DNA topology of EBV minichromosomesinHEK293cells.pdf.txtAnalysis of DNA topology of EBV minichromosomesinHEK293cells.pdf.txtExtracted texttext/plain48049http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/5/Analysis%20of%20DNA%20topology%20of%20EBV%20minichromosomesinHEK293cells.pdf.txtb8e439a7c4803fb884aee6c68a5694e6MD55THUMBNAILAnalysis of DNA topology of EBV minichromosomes in HEK 293 cells.jpgAnalysis of DNA topology of EBV minichromosomes in HEK 293 cells.jpgVista de primera página de artículo científicoimage/jpeg930979http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3345/4/Analysis%20of%20DNA%20topology%20of%20EBV%20minichromosomes%20in%20HEK%20293%20cells.jpg2f25a9f553e95879bc940ade56f9b08cMD5420.500.14066/3345oai:repositorio.conacyt.gov.py:20.500.14066/33452026-02-12 19:30:24.806Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells Castán, Alicia 6. Producción y tecnología industrial 6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations Antígenos nucleares del virus de Epstein-Barr/genética Células HEK293 Genes virales Herpesvirus humano 4 Humanos Epstein-Barr virus nuclear antigens/genetics HEK293 Cells Genes, viral Herpesvirus 4, Human/genetics Humans |
| status_str | publishedVersion |
| title | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| title_full | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| title_fullStr | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| title_full_unstemmed | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| title_short | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| title_sort | Analysis of DNA topology of EBV minichromosomes in HEK 293 cells |
| topic | 6. Producción y tecnología industrial 6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations Antígenos nucleares del virus de Epstein-Barr/genética Células HEK293 Genes virales Herpesvirus humano 4 Humanos Epstein-Barr virus nuclear antigens/genetics HEK293 Cells Genes, viral Herpesvirus 4, Human/genetics Humans |
| url | https://doi.org/10.1371/journal.pone.0188172 http://hdl.handle.net/20.500.14066/3345 |