Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay
Correspondence: m.rodriguez@cedivep.com.py; Tel.: +595-984179-025 (ext. 111422).
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| Andre forfattere: | , , , |
| Format: | article |
| Sprog: | engelsk |
| Udgivet: |
2025
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| Online adgang: | https://doi.org/10.3390/v17101295 http://hdl.handle.net/20.500.14066/4663 |
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| _version_ | 1870612065333280768 |
|---|---|
| author | Rodríguez Valinotti, María Fátima |
| author2 | Nara Pereira, Eva Megumi Martínez Pereira, Iara Magaly Rodríguez Valinotti, Rosmary Rodríguez Sánchez, Antonio |
| author2_role | author author author author |
| author_browse | Martínez Pereira, Iara Magaly Nara Pereira, Eva Megumi Rodríguez Sánchez, Antonio Rodríguez Valinotti, María Fátima Rodríguez Valinotti, Rosmary |
| author_facet | Rodríguez Valinotti, María Fátima Nara Pereira, Eva Megumi Martínez Pereira, Iara Magaly Rodríguez Valinotti, Rosmary Rodríguez Sánchez, Antonio |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/1/Molecular%20detection%20and%20characterization%20of%20bovine%20diarrhea%20virus%20%28BVDV%29%20in%20aborted%20fetuses%20and%20semen%20samples%20from%20Paraguay.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/4/Molecular%20detection%20and%20characterization%20of%20bovine%20diarrhea%20virus%20%28BVDV%29%20in%20aborted%20fetuses%20and%20semen%20samples%20from%20Paraguay.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/5/Molecular%20detection%20and%20characterization%20of%20bovine%20diarrhea%20virus%20%28BVDV%29%20in%20aborted%20fetuses%20and%20semen%20samples%20from%20Paraguay.pdf.txt |
| dc.contributor.other.es.fl_str_mv | Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud |
| dc.creator.none.fl_str_mv | Rodríguez Valinotti, María Fátima Nara Pereira, Eva Megumi Martínez Pereira, Iara Magaly Rodríguez Valinotti, Rosmary Rodríguez Sánchez, Antonio |
| dc.date.accessioned.none.fl_str_mv | 2025-10-10T12:40:44Z |
| dc.date.available.none.fl_str_mv | 2025-10-10T12:40:44Z |
| dc.date.issued.none.fl_str_mv | 2025-09-24 |
| dc.format.extent.es.fl_str_mv | 10 páginas |
| dc.identifier.citation.en.fl_str_mv | Rodriguez Valinotti, M. F., Nara Pereira, E. M., Martinez Pereira, M., Rodriguez Valinotti, R., & Rodriguez Sanchez, A. (2025). Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay. Viruses, 17(10), Article 1295. https://doi.org/10.3390/v17101295 |
| dc.identifier.doi.es.fl_str_mv | 10.3390/v17101295 |
| dc.identifier.essn.es.fl_str_mv | 1999-4915 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.3390/v17101295 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4663 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| dc.relation.projectCONACYT.es.fl_str_mv | POSG17-59 |
| dc.rights.*.fl_str_mv | Atribución/Reconocimiento 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.en.fl_str_mv | Livestock-raising Flaviviridae Cattle PCR Pestivirus Bovine diarrhoea pestivirus |
| dc.subject.es.fl_str_mv | Cría de ganado Flaviviridae Ganado bovino PCR Pestivirus Pestivirus de la diarrea bovina |
| dc.subject.other.es.fl_str_mv | BVDV Paraguay Pestivirus RT-PCR |
| dc.title.es.fl_str_mv | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Correspondence: m.rodriguez@cedivep.com.py; Tel.: +595-984179-025 (ext. 111422). |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_15dc13adfb26f492535dcbcbd4647bac |
| identifier_str_mv | Rodriguez Valinotti, M. F., Nara Pereira, E. M., Martinez Pereira, M., Rodriguez Valinotti, R., & Rodriguez Sanchez, A. (2025). Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay. Viruses, 17(10), Article 1295. https://doi.org/10.3390/v17101295 10.3390/v17101295 1999-4915 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4663 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Atribución/Reconocimiento 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| spelling | 14706000000-0003-0671-449608fa93cb-6f99-4890-9ba4-caf2a4749f936007106005f3ab3fe-0e7a-4b55-89a1-d5bb696c0d43600662c214f-8954-4eb2-ba01-fd38e93f53d0600Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud2025-10-10T12:40:44Z2025-10-10T12:40:44Z2025-09-24Rodriguez Valinotti, M. F., Nara Pereira, E. M., Martinez Pereira, M., Rodriguez Valinotti, R., & Rodriguez Sanchez, A. (2025). Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay. Viruses, 17(10), Article 1295. https://doi.org/10.3390/v17101295https://doi.org/10.3390/v17101295http://hdl.handle.net/20.500.14066/466310.3390/v171012951999-4915Correspondence: m.rodriguez@cedivep.com.py; Tel.: +595-984179-025 (ext. 111422).This article belongs to the Special Issue Bovine Viral Diarrhea Viruses and Other Pestiviruses.Bovine viral diarrhea virus (BVDV) is the most prevalent pathogen in cattle and causes significant economic losses due to its severe clinical manifestations. It belongs to the family Flaviviridae and is distributed in species A, B, and H within the genus Pestivirus. The objective of this study was to detect and characterize BVDV using molecular techniques (RT-PCR) in semen and aborted fetuses samples that were sent to the CEDIVEP (Veterinary Diagnostic Center of Paraguay) laboratory. Seventy-three samples of semen from bulls were analyzed, and 54.7% of the samples were positive for Pestivirus A. The presence of Pestivirus A and H was detected in 2/8 spontaneously aborted fetuses. The genotypes of four individual samples of type A and four samples of type H organs were confirmed by partial sequencing of the 5-UTR region. The presence of BVDV was confirmed by molecular techniques for the first time in our country through its detection in different types of samples, as well as the presence of two genotypes. This suggests that the circulation of this virus can cause significant losses in cattle production in Paraguay.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de creación y fortalecimiento de maestrías y doctorados de excelencia10 páginasengMultidisciplinary Digital Publishing InstituteAtribución/Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).Cría de ganadoFlaviviridaeGanado bovinoPCRPestivirusPestivirus de la diarrea bovinaLivestock-raisingFlaviviridaeCattlePCRPestivirusBovine diarrhoea pestivirusBVDVParaguayPestivirus RT-PCRMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguayinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10VirusesPOSG17-5917Rodríguez Valinotti, María FátimaNara Pereira, Eva MegumiMartínez Pereira, Iara MagalyRodríguez Valinotti, RosmaryRodríguez Sánchez, AntonioORIGINALMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay.pdfMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay.pdfArtículo científicoapplication/pdf539895http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/1/Molecular%20detection%20and%20characterization%20of%20bovine%20diarrhea%20virus%20%28BVDV%29%20in%20aborted%20fetuses%20and%20semen%20samples%20from%20Paraguay.pdfd7c6e22c173fa2ddd2ff50341d41cd4eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/2/license_rdf0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay.jpgMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay.jpgVista de primera página de artículo científicoimage/jpeg939290http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/4/Molecular%20detection%20and%20characterization%20of%20bovine%20diarrhea%20virus%20%28BVDV%29%20in%20aborted%20fetuses%20and%20semen%20samples%20from%20Paraguay.jpg591ec817c2aa6fa06a219f13c085ecfdMD54TEXTMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay.pdf.txtMolecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay.pdf.txtExtracted texttext/plain33911http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4663/5/Molecular%20detection%20and%20characterization%20of%20bovine%20diarrhea%20virus%20%28BVDV%29%20in%20aborted%20fetuses%20and%20semen%20samples%20from%20Paraguay.pdf.txt67b26e79a96033410eb5c2bb3be31e3cMD5520.500.14066/4663oai:repositorio.conacyt.gov.py:20.500.14066/46632026-02-12 19:30:43.912Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay Rodríguez Valinotti, María Fátima Cría de ganado Flaviviridae Ganado bovino PCR Pestivirus Pestivirus de la diarrea bovina Livestock-raising Flaviviridae Cattle PCR Pestivirus Bovine diarrhoea pestivirus BVDV Paraguay Pestivirus RT-PCR |
| status_str | publishedVersion |
| title | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| title_full | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| title_fullStr | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| title_full_unstemmed | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| title_short | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| title_sort | Molecular detection and characterization of bovine diarrhea virus (BVDV) in aborted fetuses and semen samples from Paraguay |
| topic | Cría de ganado Flaviviridae Ganado bovino PCR Pestivirus Pestivirus de la diarrea bovina Livestock-raising Flaviviridae Cattle PCR Pestivirus Bovine diarrhoea pestivirus BVDV Paraguay Pestivirus RT-PCR |
| url | https://doi.org/10.3390/v17101295 http://hdl.handle.net/20.500.14066/4663 |