Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children
Address correspondence to Fátima Rodríguez, frodriguez@iics.una.py
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| Altres autors: | , , , , , , , |
| Format: | article |
| Idioma: | anglès |
| Publicat: |
2024
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| Accés en línia: | https://doi.org/10.1128/spectrum.03012-23 http://hdl.handle.net/20.500.14066/4390 |
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| _version_ | 1870612066235056128 |
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| author | Guillén Fretes, Rosa María |
| author2 | Salinas, Claudia Mendoza Álvarez, Alejandro Rubio Rodríguez, Luis A. Díaz de Usera, Ana Lorenzo Salazar, José M. González Montelongo, Rafaela Flores, Carlos Rodríguez Acosta, Fátima |
| author2_role | author author author author author author author author |
| author_browse | Díaz de Usera, Ana Flores, Carlos González Montelongo, Rafaela Guillén Fretes, Rosa María Lorenzo Salazar, José M. Mendoza Álvarez, Alejandro Rodríguez Acosta, Fátima Rubio Rodríguez, Luis A. Salinas, Claudia |
| author_facet | Guillén Fretes, Rosa María Salinas, Claudia Mendoza Álvarez, Alejandro Rubio Rodríguez, Luis A. Díaz de Usera, Ana Lorenzo Salazar, José M. González Montelongo, Rafaela Flores, Carlos Rodríguez Acosta, Fátima |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 MD5 MD5 MD5 MD5 |
| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/1/Genomic%20epidemiology%20of%20the%20primary%20methicillin-resistant.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/10/Material%20complementario.zip http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/4/Genomic%20epidemiology%20of%20the%20primary%20methicillin-resistant.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/9/Genomic%20epidemiology%20of%20the%20primary%20methicillin-resistant.pdf.txt |
| dc.contributor.editor.es.fl_str_mv | Vignoli, Rafael |
| dc.creator.none.fl_str_mv | Guillén Fretes, Rosa María Salinas, Claudia Mendoza Álvarez, Alejandro Rubio Rodríguez, Luis A. Díaz de Usera, Ana Lorenzo Salazar, José M. González Montelongo, Rafaela Flores, Carlos Rodríguez Acosta, Fátima |
| dc.date.accessioned.none.fl_str_mv | 2024-03-19T13:39:45Z |
| dc.date.available.none.fl_str_mv | 2024-03-19T13:39:45Z |
| dc.date.issued.none.fl_str_mv | 2024-02-28 |
| dc.format.extent.es.fl_str_mv | 17 páginas |
| dc.identifier.citation.es.fl_str_mv | Guillén, R., Salinas, C., Mendoza Álvarez, A., Rubio Rodríguez, L. A., Díaz de Usera, A., Lorenzo Salazar, J. M., González Montelongo, R., Flores, C., & Rodríguez, F. (2024). Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children. Microbiology Spectrum, e03012-23. https://doi.org/10.1128/spectrum.03012-23 |
| dc.identifier.doi.es.fl_str_mv | 10.1128/spectrum.03012-23 |
| dc.identifier.essn.es.fl_str_mv | 2165-0497 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.1128/spectrum.03012-23 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4390 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | American Society for Microbiology |
| dc.relation.projectCONACYT.es.fl_str_mv | PVCT18-61 |
| dc.rights.*.fl_str_mv | Atribución 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.classification.es.fl_str_mv | 7. Salud |
| dc.subject.other.es.fl_str_mv | Genomic epidemiology Invasive infection Methicillin-resistant Staphylococcus aureus Paraguayan children |
| dc.title.es.fl_str_mv | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Address correspondence to Fátima Rodríguez, frodriguez@iics.una.py |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_2a825b698bdf7fc4b0c4ad0a0891b3ed |
| identifier_str_mv | Guillén, R., Salinas, C., Mendoza Álvarez, A., Rubio Rodríguez, L. A., Díaz de Usera, A., Lorenzo Salazar, J. M., González Montelongo, R., Flores, C., & Rodríguez, F. (2024). Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children. Microbiology Spectrum, e03012-23. https://doi.org/10.1128/spectrum.03012-23 10.1128/spectrum.03012-23 2165-0497 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4390 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| spelling | 5746000000-0002-4778-496002e740e2-1af0-4520-addc-b4d62cd2546360037931385-0bec-4cb0-8a14-5d672d07b52c6006f26a390-89a4-405d-a583-4077326febb56005d57f541-a862-469a-8da5-b41d782d04af60071be47b6-909e-4a36-836e-7d2109851a08600ab498e74-322e-4885-8ab4-60061050cfb26008ba13f3f-fbf0-4116-9866-339badcb530c600981600Vignoli, Rafael2024-03-19T13:39:45Z2024-03-19T13:39:45Z2024-02-28Guillén, R., Salinas, C., Mendoza Álvarez, A., Rubio Rodríguez, L. A., Díaz de Usera, A., Lorenzo Salazar, J. M., González Montelongo, R., Flores, C., & Rodríguez, F. (2024). Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children. Microbiology Spectrum, e03012-23. https://doi.org/10.1128/spectrum.03012-23https://doi.org/10.1128/spectrum.03012-23http://hdl.handle.net/20.500.14066/439010.1128/spectrum.03012-232165-0497Address correspondence to Fátima Rodríguez, frodriguez@iics.una.pyMethicillin-resistant Staphylococcus aureus (MRSA) is one of the major human pathogens. It could carry numerous resistance genes and virulence factors in its genome, some of which are related to the severity of the infection. An observational, descriptive, cross-sectional study was designed to molecularly analyze MRSA isolates that cause invasive infections in Paraguayan children from 2009 to 2013. Ten representative MRSA isolates of the main clonal complex identified were analyzed with short-read paired-end sequencing and assessed for the virulome, resistome, and phylogenetic relationships. All the genetically linked MRSA isolates were recovered from diverse clinical sources, patients, and hospitals at broad gap periods. The pan-genomic analysis of these clones revealed three major and different clonal complexes (CC30, CC5, and CC8), each composed of clones closely related to each other. The CC30 genomes prove to be a successful clone, strongly installed and disseminated throughout our country, and closely related to other CC30 public genomes from the region and the world. The CC5 shows the highest genetic variability, and the CC8 carried the complete arginine catabolic mobile element (ACME), closely related to the USA300-NAE-ACME+, identified as the major cause of CA-MRSA infections in North America. Multiple virulence and resistance genes were identified for the first time in this study, highlighting the complex virulence profiles of MRSA circulating in the country. This study opens a wide range of new possibilities for future projects and trials to improve the existing knowledge on the epidemiology of MRSA circulating in Paraguay.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Financiamiento para la vinculación de científicos y tecnólogos17 páginasengAmerican Society for MicrobiologyAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2024 Guillén et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.7. SaludGenomic epidemiologyInvasive infectionMethicillin-resistant Staphylococcus aureusParaguayan childrenGenomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan childreninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionMicrobiology SpectrumPVCT18-61Guillén Fretes, Rosa MaríaSalinas, ClaudiaMendoza Álvarez, AlejandroRubio Rodríguez, Luis A.Díaz de Usera, AnaLorenzo Salazar, José M.González Montelongo, RafaelaFlores, CarlosRodríguez Acosta, FátimaORIGINALGenomic epidemiology of the primary methicillin-resistant.pdfGenomic epidemiology of the primary methicillin-resistant.pdfArtículo científicoapplication/pdf861686http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/1/Genomic%20epidemiology%20of%20the%20primary%20methicillin-resistant.pdfbb8c82c14515fe9eda5a24e030b6b82dMD51Material complementario.zipMaterial complementario.zipTablas complementariasapplication/octet-stream50231http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/10/Material%20complementario.zip89851b47061967e6554c41023e1eed24MD510CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/2/license_rdf0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILGenomic epidemiology of the primary methicillin-resistant.jpgGenomic epidemiology of the primary methicillin-resistant.jpgPortadaimage/jpeg904867http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/4/Genomic%20epidemiology%20of%20the%20primary%20methicillin-resistant.jpgd777353cb2d50c83e705bae6a72d248bMD54TEXTGenomic epidemiology of the primary methicillin-resistant.pdf.txtGenomic epidemiology of the primary methicillin-resistant.pdf.txtExtracted texttext/plain69921http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4390/9/Genomic%20epidemiology%20of%20the%20primary%20methicillin-resistant.pdf.txt38399903b87a9b34831f905492af0adeMD5920.500.14066/4390oai:repositorio.conacyt.gov.py:20.500.14066/43902026-02-12 19:30:35.742Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children Guillén Fretes, Rosa María 7. Salud Genomic epidemiology Invasive infection Methicillin-resistant Staphylococcus aureus Paraguayan children |
| status_str | publishedVersion |
| title | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| title_full | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| title_fullStr | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| title_full_unstemmed | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| title_short | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| title_sort | Genomic epidemiology of the primary methicillin-resistant Staphylococcus aureus clones causing invasive infections in Paraguayan children |
| topic | 7. Salud Genomic epidemiology Invasive infection Methicillin-resistant Staphylococcus aureus Paraguayan children |
| url | https://doi.org/10.1128/spectrum.03012-23 http://hdl.handle.net/20.500.14066/4390 |