CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
Corresponding author: Dora B. Krimer, dbkrimer@cib.csic.es.
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| Other Authors: | , , , |
| Format: | article |
| Language: | English |
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2019
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| Online Access: | https://doi.org/10.7717/peerj.6284 http://hdl.handle.net/20.500.14066/3848 |
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| _version_ | 1870612066900901888 |
|---|---|
| author | Fernández Calleja, Vanessa |
| author2 | Fernández de Nestosa, María José Hernández, Pablo Schvartzman, Jorge Bernardo Krimer, Dora B. |
| author2_role | author author author author |
| author_browse | Fernández Calleja, Vanessa Fernández de Nestosa, María José Hernández, Pablo Krimer, Dora B. Schvartzman, Jorge Bernardo |
| author_facet | Fernández Calleja, Vanessa Fernández de Nestosa, María José Hernández, Pablo Schvartzman, Jorge Bernardo Krimer, Dora B. |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/1/CRISPR-Cas9-mediated%20deletion%20of%20the%20Wiskott-Aldrich%20syndrome%20locus%20causes%20actin%20cytoskeleton%20disorganization%20in%20murine%20erythroleukemia%20cells.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/6/Supplemental%20Information.zip http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/2/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/3/PINV15-573art4.pdf.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/4/CRISPR/Cas9-mediated%20deletion%20of%20the%20Wiskott-Aldrich%20syndrome%20locus%20causes%20actin%20cytoskeleton%20disorganization%20in%20murine%20erythroleukemia%20cells.pdf.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/7/CRISPR-Cas9-mediated%20deletion%20of%20the%20Wiskott-Aldrich%20syndrome%20locus%20causes%20actin%20cytoskeleton%20disorganization%20in%20murine%20erythroleukemia%20cells.pdf.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/5/CRISPR-Cas9-mediated%20deletion%20of%20the%20Wiskott-Aldrich%20syndrome%20locus%20causes.jpg |
| dc.contributor.other.es.fl_str_mv | Universidad Nacional de Asunción. Facultad Politécnica |
| dc.creator.none.fl_str_mv | Fernández Calleja, Vanessa Fernández de Nestosa, María José Hernández, Pablo Schvartzman, Jorge Bernardo Krimer, Dora B. |
| dc.date.accessioned.none.fl_str_mv | 2022-05-02T18:48:43Z |
| dc.date.available.none.fl_str_mv | 2022-05-02T18:48:43Z |
| dc.date.issued.none.fl_str_mv | 2019-01-16 |
| dc.format.extent.es.fl_str_mv | 18 páginas |
| dc.identifier.citation.en.fl_str_mv | Fernández Calleja, V., Fernández Nestosa, M. J., Hernández, P., Schvartzman, J. B., & Krimer, D. B. (2019). CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. PeerJ, Artículo 7:e6284. https://doi.org/10.7717/peerj.6284 |
| dc.identifier.doi.es.fl_str_mv | 10.7717/peerj.6284 |
| dc.identifier.essn.es.fl_str_mv | 2167-8359 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.7717/peerj.6284 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/3848 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.en.fl_str_mv | PeerJ, Inc. |
| dc.relation.projectCONACYT.es.fl_str_mv | PINV15-573 |
| dc.rights.*.fl_str_mv | Attribution 4.0 International |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.copyright.es.fl_str_mv | © 2019 Fernández-Calleja et al. |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.classification.es.fl_str_mv | 7. Salud 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles |
| dc.subject.en.fl_str_mv | Hematology Molecular Biology |
| dc.subject.ocde.es.fl_str_mv | 1. Ciencias Naturales 1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)] |
| dc.subject.other.es.fl_str_mv | Actin cytoskeleton Bruton tyrosine kinase CRISPR/Cas9 Erythroleukemia cells Wiskott-Aldrich |
| dc.title.es.fl_str_mv | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Corresponding author: Dora B. Krimer, dbkrimer@cib.csic.es. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_3b6035f0bf8e5ec00b750d88505b8377 |
| identifier_str_mv | Fernández Calleja, V., Fernández Nestosa, M. J., Hernández, P., Schvartzman, J. B., & Krimer, D. B. (2019). CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. PeerJ, Artículo 7:e6284. https://doi.org/10.7717/peerj.6284 10.7717/peerj.6284 2167-8359 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/3848 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ © 2019 Fernández-Calleja et al. |
| spelling | a355d0b0-23b4-480f-9273-6490411036b9600411600644c6599-893c-4718-9a69-f27695fa83916001065600a0699453-d881-4d8a-ada8-538c5042aaaf600Universidad Nacional de Asunción. Facultad Politécnica2022-05-02T18:48:43Z2022-05-02T18:48:43Z2019-01-16Fernández Calleja, V., Fernández Nestosa, M. J., Hernández, P., Schvartzman, J. B., & Krimer, D. B. (2019). CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. PeerJ, Artículo 7:e6284. https://doi.org/10.7717/peerj.6284https://doi.org/10.7717/peerj.6284http://hdl.handle.net/20.500.14066/384810.7717/peerj.62842167-8359Corresponding author: Dora B. Krimer, dbkrimer@cib.csic.es.Wiskott-Aldrich syndrome (WAS) is a recessive X-linked inmmunodeficiency caused by loss-of-function mutations in the gene encoding the WAS protein (WASp). WASp plays an important role in the polymerization of the actin cytoskeleton in hematopoietic cells through activation of the Arp2/3 complex. In a previous study, we found that actin cytoskeleton proteins, including WASp, were silenced in murine erythroleukemia cells defective in differentiation. Here, we designed a CRISPR/Cas9 strategy to delete a 9.5-kb genomic region encompassing the Was gene in the X chromosome of murine erythroleukemia (MEL) cells. We show that Was-deficient MEL cells have a poor organization of the actin cytoskeleton that can be recovered by restoring Was expression. We found that whereas the total amount of actin protein was similar between wild-type and Was knockout MEL cells, the latter exhibited an altered ratio of monomeric G-actin to polymeric F-actin. We also demonstrate that Was overexpression can mediate the activation of Bruton’s tyrosine kinase. Overall, these findings support the role of WASp as a key regulator of F-actin in erythroid cells.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrollo18 páginasengPeerJ, Inc.Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2019 Fernández-Calleja et al.HematologyMolecular Biology7. Salud7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisiblesActin cytoskeletonBruton tyrosine kinaseCRISPR/Cas9Erythroleukemia cellsWiskott-Aldrich1. Ciencias Naturales1.1. 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| spellingShingle | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells Fernández Calleja, Vanessa Hematology Molecular Biology 7. Salud 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles Actin cytoskeleton Bruton tyrosine kinase CRISPR/Cas9 Erythroleukemia cells Wiskott-Aldrich 1. Ciencias Naturales 1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)] |
| status_str | publishedVersion |
| title | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| title_full | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| title_fullStr | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| title_full_unstemmed | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| title_short | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| title_sort | CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells |
| topic | Hematology Molecular Biology 7. Salud 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles Actin cytoskeleton Bruton tyrosine kinase CRISPR/Cas9 Erythroleukemia cells Wiskott-Aldrich 1. Ciencias Naturales 1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)] |
| url | https://doi.org/10.7717/peerj.6284 http://hdl.handle.net/20.500.14066/3848 |