CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells

Corresponding author: Dora B. Krimer, dbkrimer@cib.csic.es.

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Main Author: Fernández Calleja, Vanessa (author)
Other Authors: Fernández de Nestosa, María José (author), Hernández, Pablo (author), Schvartzman, Jorge Bernardo (author), Krimer, Dora B. (author)
Format: article
Language:English
Published: 2019
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Online Access:https://doi.org/10.7717/peerj.6284
http://hdl.handle.net/20.500.14066/3848
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author Fernández Calleja, Vanessa
author2 Fernández de Nestosa, María José
Hernández, Pablo
Schvartzman, Jorge Bernardo
Krimer, Dora B.
author2_role author
author
author
author
author_browse Fernández Calleja, Vanessa
Fernández de Nestosa, María José
Hernández, Pablo
Krimer, Dora B.
Schvartzman, Jorge Bernardo
author_facet Fernández Calleja, Vanessa
Fernández de Nestosa, María José
Hernández, Pablo
Schvartzman, Jorge Bernardo
Krimer, Dora B.
author_role author
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dc.contributor.other.es.fl_str_mv Universidad Nacional de Asunción. Facultad Politécnica
dc.creator.none.fl_str_mv Fernández Calleja, Vanessa
Fernández de Nestosa, María José
Hernández, Pablo
Schvartzman, Jorge Bernardo
Krimer, Dora B.
dc.date.accessioned.none.fl_str_mv 2022-05-02T18:48:43Z
dc.date.available.none.fl_str_mv 2022-05-02T18:48:43Z
dc.date.issued.none.fl_str_mv 2019-01-16
dc.format.extent.es.fl_str_mv 18 páginas
dc.identifier.citation.en.fl_str_mv Fernández Calleja, V., Fernández Nestosa, M. J., Hernández, P., Schvartzman, J. B., & Krimer, D. B. (2019). CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. PeerJ, Artículo 7:e6284. https://doi.org/10.7717/peerj.6284
dc.identifier.doi.es.fl_str_mv 10.7717/peerj.6284
dc.identifier.essn.es.fl_str_mv 2167-8359
dc.identifier.other.es.fl_str_mv https://doi.org/10.7717/peerj.6284
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.14066/3848
dc.language.iso.es.fl_str_mv eng
dc.publisher.en.fl_str_mv PeerJ, Inc.
dc.relation.projectCONACYT.es.fl_str_mv PINV15-573
dc.rights.*.fl_str_mv Attribution 4.0 International
dc.rights.accessRights.es.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.copyright.es.fl_str_mv © 2019 Fernández-Calleja et al.
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.subject.classification.es.fl_str_mv 7. Salud
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
dc.subject.en.fl_str_mv Hematology
Molecular Biology
dc.subject.ocde.es.fl_str_mv 1. Ciencias Naturales
1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)]
dc.subject.other.es.fl_str_mv Actin cytoskeleton
Bruton tyrosine kinase
CRISPR/Cas9
Erythroleukemia cells
Wiskott-Aldrich
dc.title.es.fl_str_mv CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
dc.type.es.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description Corresponding author: Dora B. Krimer, dbkrimer@cib.csic.es.
eu_rights_str_mv openAccess
format article
id CONACYT_3b6035f0bf8e5ec00b750d88505b8377
identifier_str_mv Fernández Calleja, V., Fernández Nestosa, M. J., Hernández, P., Schvartzman, J. B., & Krimer, D. B. (2019). CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. PeerJ, Artículo 7:e6284. https://doi.org/10.7717/peerj.6284
10.7717/peerj.6284
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language eng
network_acronym_str CONACYT
network_name_str Repositorio Institucional CONACYT
oai_identifier_str oai:repositorio.conacyt.gov.py:20.500.14066/3848
publishDate 2019
publishDateSort 2019
repository.mail.fl_str_mv repositorio.institucional@conacyt.gov.py
repository.name.fl_str_mv Repositorio Institucional CONACYT
repository_id_str
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
© 2019 Fernández-Calleja et al.
spelling a355d0b0-23b4-480f-9273-6490411036b9600411600644c6599-893c-4718-9a69-f27695fa83916001065600a0699453-d881-4d8a-ada8-538c5042aaaf600Universidad Nacional de Asunción. Facultad Politécnica2022-05-02T18:48:43Z2022-05-02T18:48:43Z2019-01-16Fernández Calleja, V., Fernández Nestosa, M. J., Hernández, P., Schvartzman, J. B., & Krimer, D. B. (2019). CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells. PeerJ, Artículo 7:e6284. https://doi.org/10.7717/peerj.6284https://doi.org/10.7717/peerj.6284http://hdl.handle.net/20.500.14066/384810.7717/peerj.62842167-8359Corresponding author: Dora B. Krimer, dbkrimer@cib.csic.es.Wiskott-Aldrich syndrome (WAS) is a recessive X-linked inmmunodeficiency caused by loss-of-function mutations in the gene encoding the WAS protein (WASp). WASp plays an important role in the polymerization of the actin cytoskeleton in hematopoietic cells through activation of the Arp2/3 complex. In a previous study, we found that actin cytoskeleton proteins, including WASp, were silenced in murine erythroleukemia cells defective in differentiation. Here, we designed a CRISPR/Cas9 strategy to delete a 9.5-kb genomic region encompassing the Was gene in the X chromosome of murine erythroleukemia (MEL) cells. We show that Was-deficient MEL cells have a poor organization of the actin cytoskeleton that can be recovered by restoring Was expression. We found that whereas the total amount of actin protein was similar between wild-type and Was knockout MEL cells, the latter exhibited an altered ratio of monomeric G-actin to polymeric F-actin. We also demonstrate that Was overexpression can mediate the activation of Bruton’s tyrosine kinase. Overall, these findings support the role of WASp as a key regulator of F-actin in erythroid cells.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrollo18 páginasengPeerJ, Inc.Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2019 Fernández-Calleja et al.HematologyMolecular Biology7. Salud7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisiblesActin cytoskeletonBruton tyrosine kinaseCRISPR/Cas9Erythroleukemia cellsWiskott-Aldrich1. Ciencias Naturales1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)]CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cellsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeerJPINV15-5737Fernández Calleja, VanessaFernández de Nestosa, María JoséHernández, PabloSchvartzman, Jorge BernardoKrimer, Dora B.ORIGINALCRISPR-Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells.pdfCRISPR-Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells.pdfArtículo científicoapplication/pdf12873984http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/1/CRISPR-Cas9-mediated%20deletion%20of%20the%20Wiskott-Aldrich%20syndrome%20locus%20causes%20actin%20cytoskeleton%20disorganization%20in%20murine%20erythroleukemia%20cells.pdfd2b8e7f134b89ee3f00575fbd96cb3d2MD51Supplemental Information.zipSupplemental Information.zipInformación complementariaapplication/octet-stream16939957http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3848/6/Supplemental%20Information.zip073cb1e3282f5adca40f8e567cfbc46dMD56LICENSElicense.txtlicense.txttext/plain; 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spellingShingle CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
Fernández Calleja, Vanessa
Hematology
Molecular Biology
7. Salud
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
Actin cytoskeleton
Bruton tyrosine kinase
CRISPR/Cas9
Erythroleukemia cells
Wiskott-Aldrich
1. Ciencias Naturales
1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)]
status_str publishedVersion
title CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
title_full CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
title_fullStr CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
title_full_unstemmed CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
title_short CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
title_sort CRISPR/Cas9-mediated deletion of the Wiskott-Aldrich syndrome locus causes actin cytoskeleton disorganization in murine erythroleukemia cells
topic Hematology
Molecular Biology
7. Salud
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
Actin cytoskeleton
Bruton tyrosine kinase
CRISPR/Cas9
Erythroleukemia cells
Wiskott-Aldrich
1. Ciencias Naturales
1.1. Matemáticas e Informática [matemáticas y otras áreas afines; informática y otras disciplinas afines (solo desarrollo de software; el desarrollo de equipos debe clasificarse en ingeniería)]
url https://doi.org/10.7717/peerj.6284
http://hdl.handle.net/20.500.14066/3848