Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay

Correspondence: Nilsa González Brítez; gbritez.nilsa@gmail.com

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Autor principal: Brítez López, Sady Camila (author)
Altres autors: Herrera Cabrera, Leidi Maira (author), Ferreira, María C. (author), Rolón, Leticia M. (author), Ruíz, Vanessa (author), González Brítez, Nilsa Elizabeth (author)
Format: article
Idioma:anglès
Publicat: 2022
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Accés en línia:https://doi.org/10.17843/rpmesp.2022.392.10709
http://hdl.handle.net/20.500.14066/4374
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author Brítez López, Sady Camila
author2 Herrera Cabrera, Leidi Maira
Ferreira, María C.
Rolón, Leticia M.
Ruíz, Vanessa
González Brítez, Nilsa Elizabeth
author2_role author
author
author
author
author
author_browse Brítez López, Sady Camila
Ferreira, María C.
González Brítez, Nilsa Elizabeth
Herrera Cabrera, Leidi Maira
Rolón, Leticia M.
Ruíz, Vanessa
author_facet Brítez López, Sady Camila
Herrera Cabrera, Leidi Maira
Ferreira, María C.
Rolón, Leticia M.
Ruíz, Vanessa
González Brítez, Nilsa Elizabeth
author_role author
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dc.contributor.other.es.fl_str_mv Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud
Asociación de Funcionarios, Docentes e Investigadores de la Universidad Nacional de Caaguazú
dc.creator.none.fl_str_mv Brítez López, Sady Camila
Herrera Cabrera, Leidi Maira
Ferreira, María C.
Rolón, Leticia M.
Ruíz, Vanessa
González Brítez, Nilsa Elizabeth
dc.date.accessioned.none.fl_str_mv 2024-02-27T15:29:09Z
dc.date.available.none.fl_str_mv 2024-02-27T15:29:09Z
dc.date.issued.none.fl_str_mv 2022-06-30
dc.identifier.citation.en.fl_str_mv Brítez, S. C., Herrera, L., Ferreira, M. C., Rolón, L. M., Ruiz, V., & González Brítez, N. (2022). Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay. Revista Peruana de Medicina Experimental y Salud Pública, 39(2), 170-7. https://doi.org/10.17843/rpmesp.2022.392.10709
dc.identifier.doi.es.fl_str_mv 10.17843/rpmesp.2022.392.10709
dc.identifier.essn.es.fl_str_mv 1726-4642
dc.identifier.issn.es.fl_str_mv 1726-4634
dc.identifier.other.*.fl_str_mv https://doi.org/10.17843/rpmesp.2022.392.10709
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.14066/4374
dc.language.iso.es.fl_str_mv eng
dc.publisher.es.fl_str_mv Instituto Nacional de Salud
dc.relation.projectCONACYT.es.fl_str_mv PINV15-777
PINV15-704
dc.relation.url.es.fl_str_mv http://hdl.handle.net/20.500.14066/4370
dc.rights.*.fl_str_mv Atribución 4.0 Internacional
dc.rights.accessRights.es.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.copyright.es.fl_str_mv © 2022 Revista Peruana de Medicina Experimental y Salud Pública
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.subject.classification.es.fl_str_mv 7. Salud
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
dc.subject.ocde.es.fl_str_mv 3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología)
dc.subject.other.es.fl_str_mv Aedes
Genetic polymorphism
Mosquito vectors
Paraguay
Random Amplified Polymorphic DNA Technique
Surveillance
dc.title.es.fl_str_mv Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
dc.type.es.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description Correspondence: Nilsa González Brítez; gbritez.nilsa@gmail.com
eu_rights_str_mv openAccess
format article
id CONACYT_434f93348cf058d138d77a4acfcbe9b3
identifier_str_mv Brítez, S. C., Herrera, L., Ferreira, M. C., Rolón, L. M., Ruiz, V., & González Brítez, N. (2022). Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay. Revista Peruana de Medicina Experimental y Salud Pública, 39(2), 170-7. https://doi.org/10.17843/rpmesp.2022.392.10709
1726-4634
10.17843/rpmesp.2022.392.10709
1726-4642
language eng
network_acronym_str CONACYT
network_name_str Repositorio Institucional CONACYT
oai_identifier_str oai:repositorio.conacyt.gov.py:20.500.14066/4374
publishDate 2022
publishDateSort 2022
repository.mail.fl_str_mv repositorio.institucional@conacyt.gov.py
repository.name.fl_str_mv Repositorio Institucional CONACYT
repository_id_str
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
© 2022 Revista Peruana de Medicina Experimental y Salud Pública
spelling 12246000000-0002-8573-5334588600744982d4-5d14-4b9c-8a3b-b5298af2499f600c8222e51-2f2c-4447-9fd5-4d1dd0dd7ed8600a7deec8b-65ff-45e8-80ba-005e56537dc86005266000000-0001-5562-9728Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la SaludAsociación de Funcionarios, Docentes e Investigadores de la Universidad Nacional de Caaguazú2024-02-27T15:29:09Z2024-02-27T15:29:09Z2022-06-30Brítez, S. C., Herrera, L., Ferreira, M. C., Rolón, L. M., Ruiz, V., & González Brítez, N. (2022). Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay. Revista Peruana de Medicina Experimental y Salud Pública, 39(2), 170-7. https://doi.org/10.17843/rpmesp.2022.392.107091726-4634https://doi.org/10.17843/rpmesp.2022.392.10709http://hdl.handle.net/20.500.14066/437410.17843/rpmesp.2022.392.107091726-4642Correspondence: Nilsa González Brítez; gbritez.nilsa@gmail.comObjective: To determine the genetic diversity of Aedes aegypti in the Central-Alto Paraná cross-border road corridor of Paraguay, an area that has reports of dengue cases. Materials and methods: Twenty adult females were selected from hatching Ae. aegypti eggs from households geolocated in the departments of Alto Paraná, Caaguazú, Cordillera and Central, between 2018 and 2019. DNA was extracted from the tissue of females for amplifying their polymorphic patterns by random amplification of polymorphic DNA by PCR (RAPD-PCR), using primers H3 and B03 in order to identify genetic parameters of population diversity. The relationships between mosquito populations according to locality were observed by unpaired arithmetic mean analysis. We used DIVA-GIS 7.3.0 and MAXENT to analyze the suitable areas of actual and potential geographic distribution of these Ae. aegypti populations. Results: Forty loci were identified by RAPD-PCR profiling, with moderate gene differentiation (Gst = 0.12). The cross-border corridor presented bioclimatic conditions for the presence of variant populations of Ae. aegypti, with precipitation in the warmest quarter and mean temperature in the driest quarter being determinant in the distribution. Conclusions: There is evidence of moderate genetic diversity in Ae. aegypti populations from areas that have reported dengue cases in the cross-border road corridor linking the Central and Alto Paraná departments of Paraguay. The study of genetic variability of Ae. aegypti is very useful for entomo-epidemiological surveillance and evaluation of possible resistance to chemical control.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrolloengInstituto Nacional de SaludAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2022 Revista Peruana de Medicina Experimental y Salud Pública7. Salud7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisiblesAedesGenetic polymorphismMosquito vectorsParaguayRandom Amplified Polymorphic DNA TechniqueSurveillance3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología)Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguayinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2Revista Peruana de Medicina Experimental y Salud Pública170177PINV15-777PINV15-704http://hdl.handle.net/20.500.14066/437039Brítez López, Sady CamilaHerrera Cabrera, Leidi MairaFerreira, María C.Rolón, Leticia M.Ruíz, VanessaGonzález Brítez, Nilsa ElizabethORIGINALGenetic diversity of aedes aegypti in the Central-upper.pdfGenetic diversity of aedes aegypti in the Central-upper.pdfArtículo científicoapplication/pdf350456http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4374/1/Genetic%20diversity%20of%20aedes%20aegypti%20in%20the%20Central-upper.pdf0ec17cd706c26eee31caf4c5e472b754MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4374/2/license_rdf0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4374/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILGenetic diversity of Aedes aegypti.jpgGenetic diversity of Aedes aegypti.jpgPortadaimage/jpeg822231http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4374/4/Genetic%20diversity%20of%20Aedes%20aegypti.jpged8adc844c9a934ebc6e1df2017d8a32MD54TEXTGenetic diversity of aedes aegypti in the Central-upper.pdf.txtGenetic diversity of aedes aegypti in the Central-upper.pdf.txtExtracted texttext/plain35234http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4374/5/Genetic%20diversity%20of%20aedes%20aegypti%20in%20the%20Central-upper.pdf.txt2658eb07820c975897874d0708bd3845MD5520.500.14066/4374oai:repositorio.conacyt.gov.py:20.500.14066/43742026-02-12 19:30:35.367Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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
spellingShingle Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
Brítez López, Sady Camila
7. Salud
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
Aedes
Genetic polymorphism
Mosquito vectors
Paraguay
Random Amplified Polymorphic DNA Technique
Surveillance
3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología)
status_str publishedVersion
title Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
title_full Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
title_fullStr Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
title_full_unstemmed Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
title_short Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
title_sort Genetic diversity of Aedes aegypti in the Central-Upper Paraná cross-border axis in Paraguay
topic 7. Salud
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
Aedes
Genetic polymorphism
Mosquito vectors
Paraguay
Random Amplified Polymorphic DNA Technique
Surveillance
3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología)
url https://doi.org/10.17843/rpmesp.2022.392.10709
http://hdl.handle.net/20.500.14066/4374