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Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.

The addition of molecular biomarkers is needed to increase the accuracy of pathologic factors as prognosticators of outcome in penile squamous cell carcinomas (SCC). Evaluation of these biomarkers is usually carried out by immunohistochemistry. Herein we assess p53 immunohistochemical expression on...

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Hovedforfatter: Chaux Salinas, Alcides Manuel (author)
Andre forfattere: Burnett, Authur (author), Netto, George (author)
Format: article
Sprog:engelsk
Udgivet: 2015
Fag:
1303 I+D en relación con las Ciencias médicas
CARCINOMAS
MOLECULAR BIOMARKERS
IMMUNOHISTOCHEMISTRY
DIGITAL IMAGE ANALYSIS
OPEN-SOURCE SOFTWARE
Online adgang:http://hdl.handle.net/20.500.14066/3579
https://doi.org/10.1101/029850
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author Chaux Salinas, Alcides Manuel
author2 Burnett, Authur
Netto, George
author2_role author
author
author_browse Burnett, Authur
Chaux Salinas, Alcides Manuel
Netto, George
author_facet Chaux Salinas, Alcides Manuel
Burnett, Authur
Netto, George
author_role author
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bitstream.url.fl_str_mv http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3579/1/14-INV-323art.pdf
http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3579/2/license.txt
http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3579/3/14-INV-323art.pdf.txt
dc.contributor.other.es.fl_str_mv Centro para el Desarrollo de la Investigación Científica - CEDIC (PY)
dc.creator.none.fl_str_mv Chaux Salinas, Alcides Manuel
Burnett, Authur
Netto, George
dc.date.accessioned.none.fl_str_mv 2022-04-25T14:46:11Z
dc.date.available.none.fl_str_mv 2022-04-25T14:46:11Z
dc.date.issued.none.fl_str_mv 2015
dc.identifier.doi.es.fl_str_mv https://doi.org/10.1101/029850
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.14066/3579
dc.language.iso.es.fl_str_mv eng
dc.relation.projectCONACYT.es.fl_str_mv 14-INV-323
dc.rights.accessRights.es.fl_str_mv info:eu-repo/semantics/openAccess
dc.subject.classification.es.fl_str_mv 1303 I+D en relación con las Ciencias médicas
dc.subject.other.es.fl_str_mv CARCINOMAS
MOLECULAR BIOMARKERS
IMMUNOHISTOCHEMISTRY
DIGITAL IMAGE ANALYSIS
OPEN-SOURCE SOFTWARE
dc.title.es.fl_str_mv Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
dc.type.es.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description The addition of molecular biomarkers is needed to increase the accuracy of pathologic factors as prognosticators of outcome in penile squamous cell carcinomas (SCC). Evaluation of these biomarkers is usually carried out by immunohistochemistry. Herein we assess p53 immunohistochemical expression on tissue samples of penile SCC using freely-available, open-source software packages for digital image analysis. We also compared the results of digital analysis with standard visual estimation. Percentages of p53 positive cells were higher by visual estimation than by digital analysis. However, correlation was high between both methods. Our study shows that evaluation of p53 immunohistochemical expression is feasible using open-source software packages for digital image analysis. Although our analysis was limited to penile SCC, the rationale should also hold for other tumor types in which evaluation of p53 immunohistochemical expression is required. This approach would reduce interobserver variability, and would provide a standardized method for reporting the results of immunohistochemical stains. As these diagnostic tools are freely-available online, researchers and practicing pathologists could incorporate them in their daily practice without increasing diagnostic costs.
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oai_identifier_str oai:repositorio.conacyt.gov.py:20.500.14066/3579
publishDate 2015
publishDateSort 2015
repository.mail.fl_str_mv repositorio.institucional@conacyt.gov.py
repository.name.fl_str_mv Repositorio Institucional CONACYT
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spelling 2886000000-0002-5824-98679ca61617-02f4-4e50-9f0a-fc9a281c75a260059a1e31c-fa2a-497b-a414-5e1bebb6be45600Centro para el Desarrollo de la Investigación Científica - CEDIC (PY)2022-04-25T14:46:11Z2022-04-25T14:46:11Z2015http://hdl.handle.net/20.500.14066/3579https://doi.org/10.1101/029850The addition of molecular biomarkers is needed to increase the accuracy of pathologic factors as prognosticators of outcome in penile squamous cell carcinomas (SCC). Evaluation of these biomarkers is usually carried out by immunohistochemistry. Herein we assess p53 immunohistochemical expression on tissue samples of penile SCC using freely-available, open-source software packages for digital image analysis. We also compared the results of digital analysis with standard visual estimation. Percentages of p53 positive cells were higher by visual estimation than by digital analysis. However, correlation was high between both methods. Our study shows that evaluation of p53 immunohistochemical expression is feasible using open-source software packages for digital image analysis. Although our analysis was limited to penile SCC, the rationale should also hold for other tumor types in which evaluation of p53 immunohistochemical expression is required. This approach would reduce interobserver variability, and would provide a standardized method for reporting the results of immunohistochemical stains. As these diagnostic tools are freely-available online, researchers and practicing pathologists could incorporate them in their daily practice without increasing diagnostic costs.Consejo Nacional de Ciencia y TecnologíaPROCIENCIAeng1303 I+D en relación con las Ciencias médicasCARCINOMASMOLECULAR BIOMARKERSIMMUNOHISTOCHEMISTRYDIGITAL IMAGE ANALYSISOPEN-SOURCE SOFTWAREEvaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14-INV-323info:eu-repo/semantics/openAccessChaux Salinas, Alcides ManuelBurnett, AuthurNetto, GeorgeORIGINAL14-INV-323art.pdf14-INV-323art.pdf14-INV-323artapplication/pdf1300567http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3579/1/14-INV-323art.pdf5f81daeeeb3677a60e04f3b9e0d06132MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3579/2/license.txt858b22fda432bd774e469302988c1974MD52TEXT14-INV-323art.pdf.txt14-INV-323art.pdf.txtExtracted texttext/plain16321http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3579/3/14-INV-323art.pdf.txtbdbf790b653567d08d68f810baab1bf1MD5320.500.14066/3579oai:repositorio.conacyt.gov.py:20.500.14066/35792026-02-12 19:30:28.731Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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
spellingShingle Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
Chaux Salinas, Alcides Manuel
1303 I+D en relación con las Ciencias médicas
CARCINOMAS
MOLECULAR BIOMARKERS
IMMUNOHISTOCHEMISTRY
DIGITAL IMAGE ANALYSIS
OPEN-SOURCE SOFTWARE
status_str publishedVersion
title Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
title_full Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
title_fullStr Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
title_full_unstemmed Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
title_short Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
title_sort Evaluation of p53 Immunohistochemical Expression Using Open-Source Software for Digital Image Analysis. A Tissue Microarray Study of Penile Squamous Cell Carcinomas.
topic 1303 I+D en relación con las Ciencias médicas
CARCINOMAS
MOLECULAR BIOMARKERS
IMMUNOHISTOCHEMISTRY
DIGITAL IMAGE ANALYSIS
OPEN-SOURCE SOFTWARE
url http://hdl.handle.net/20.500.14066/3579
https://doi.org/10.1101/029850

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