Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion
Correspondence: nelson@qui.una.py (N.A.); rguillen@qui.una.py (R.G.); Tel.: +595-981446181 (N.A.); +595-994318520 (R.G.).
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| Other Authors: | , , , |
| Format: | article |
| Language: | English |
| Published: |
2022
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| Online Access: | https://doi.org/10.3390/microorganisms10081527 http://hdl.handle.net/20.500.14066/4740 |
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| _version_ | 1870885384790999040 |
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| author | Salinas Dávalos, Claudia de los Ángeles |
| author2 | Florentín Candia, Gladys Melisa Rodríguez Acosta, Fátima Alvarenga Sosa, Nelson Luis Guillén Fretes, Rosa María |
| author2_role | author author author author |
| author_browse | Alvarenga Sosa, Nelson Luis Florentín Candia, Gladys Melisa Guillén Fretes, Rosa María Rodríguez Acosta, Fátima Salinas Dávalos, Claudia de los Ángeles |
| author_facet | Salinas Dávalos, Claudia de los Ángeles Florentín Candia, Gladys Melisa Rodríguez Acosta, Fátima Alvarenga Sosa, Nelson Luis Guillén Fretes, Rosa María |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/1/Terpenes%20combinations%20inhibit%20biofilm%20formation%20in%20Staphyloccocus%20aureus%20by%20interfering%20with%20initial%20adhesion.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/4/Terpenes%20combinations%20inhibit%20biofilm%20formation%20in%20Staphyloccocus%20aureus%20by%20interfering%20with%20initial%20adhesion.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/5/Terpenes%20combinations%20inhibit%20biofilm%20formation%20in%20Staphyloccocus%20aureus%20by%20interfering%20with%20initial%20adhesion.pdf.txt |
| dc.creator.none.fl_str_mv | Salinas Dávalos, Claudia de los Ángeles Florentín Candia, Gladys Melisa Rodríguez Acosta, Fátima Alvarenga Sosa, Nelson Luis Guillén Fretes, Rosa María |
| dc.date.accessioned.none.fl_str_mv | 2026-01-16T14:36:56Z |
| dc.date.available.none.fl_str_mv | 2026-01-16T14:36:56Z |
| dc.date.issued.none.fl_str_mv | 2022-07-28 |
| dc.format.extent.es.fl_str_mv | 11 páginas |
| dc.identifier.citation.en.fl_str_mv | Salinas, C., Florentín, G., Rodríguez, F., Alvarenga, N., & Guillén, R. (2022). Terpenes Combinations Inhibit Biofilm Formation in Staphyloccocus aureus by Interfering with Initial Adhesion. Microorganisms, 10(8), 1527. https://doi.org/10.3390/microorganisms10081527 |
| dc.identifier.doi.es.fl_str_mv | 10.3390/microorganisms10081527 |
| dc.identifier.issn.es.fl_str_mv | 2076-2607 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.3390/microorganisms10081527 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4740 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| dc.relation.institBenef.es.fl_str_mv | Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud |
| dc.relation.projectCONACYT.es.fl_str_mv | POSG17-59 |
| dc.rights.*.fl_str_mv | Atribución/Reconocimiento 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.other.es.fl_str_mv | Biofilm S. aureus Terpenes |
| dc.title.es.fl_str_mv | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Correspondence: nelson@qui.una.py (N.A.); rguillen@qui.una.py (R.G.); Tel.: +595-981446181 (N.A.); +595-994318520 (R.G.). |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_4d293f0124565c68091da047421e44b7 |
| identifier_str_mv | Salinas, C., Florentín, G., Rodríguez, F., Alvarenga, N., & Guillén, R. (2022). Terpenes Combinations Inhibit Biofilm Formation in Staphyloccocus aureus by Interfering with Initial Adhesion. Microorganisms, 10(8), 1527. https://doi.org/10.3390/microorganisms10081527 2076-2607 10.3390/microorganisms10081527 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4740 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Atribución/Reconocimiento 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| spelling | 12366000000-0002-8759-019113946000000-0001-7609-2608981600466000000-0002-7949-73415746000000-0002-4778-49602026-01-16T14:36:56Z2026-01-16T14:36:56Z2022-07-28Salinas, C., Florentín, G., Rodríguez, F., Alvarenga, N., & Guillén, R. (2022). Terpenes Combinations Inhibit Biofilm Formation in Staphyloccocus aureus by Interfering with Initial Adhesion. Microorganisms, 10(8), 1527. https://doi.org/10.3390/microorganisms100815272076-2607https://doi.org/10.3390/microorganisms10081527http://hdl.handle.net/20.500.14066/474010.3390/microorganisms10081527Correspondence: nelson@qui.una.py (N.A.); rguillen@qui.una.py (R.G.); Tel.: +595-981446181 (N.A.); +595-994318520 (R.G.).This article belongs to the Section Biofilm.The biofilm is a conglomerate of cells surrounded by an extracellular matrix, which contributes to the persistence of infections. The difficulty in removing the biofilm drives the research for new therapeutic options. In this work, the effect of terpenes (−)-trans-Caryophyllene, (S)-cis-Verbenol, (S)-(−)-Limonene, (R)-(+)-Limonene, and Linalool was evaluated, individually and in combinations on bacterial growth, by assay with resazurin; the formation of biofilm, by assay with violet crystal; and the expression of associated genes, by real-time PCR, in two clinical isolates of Staphyloccocus aureus, ST30-t019 and ST5-t311, responsible for more than 90% of pediatric infections by this pathogen in Paraguay. All combinations of terpenes can inhibit biofilm formation in more than 50% without affecting bacterial growth. The most effective combination was (−)-trans-Caryophyllene and Linalool at a 500 μg/mL concentration for each, with an inhibition percentage of 88%. This combination decreased the expression levels of the sdrD, spa, agr, and hld genes associated with the initial cell adhesion stage and quorum sensing. At the same time, it increased the expression levels of the cap5B and cap5C genes related to the production of capsular polysaccharides. The combinations of compounds tested are promising alternatives to inhibit biofilm formation in S. aureus.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de creación y fortalecimiento de maestrías y doctorados de excelencia11 páginasengMultidisciplinary Digital Publishing InstituteAtribución/Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).BiofilmS. aureusTerpenesTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8MicroorganismsPOSG17-59Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud10Salinas Dávalos, Claudia de los ÁngelesFlorentín Candia, Gladys MelisaRodríguez Acosta, FátimaAlvarenga Sosa, Nelson LuisGuillén Fretes, Rosa MaríaORIGINALTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion.pdfTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion.pdfArtículo científicoapplication/pdf715630http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/1/Terpenes%20combinations%20inhibit%20biofilm%20formation%20in%20Staphyloccocus%20aureus%20by%20interfering%20with%20initial%20adhesion.pdf973d39e9b7ffd2d1eb54e1ef3f7bbe21MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/2/license_rdf0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion.jpgTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion.jpgArtículo científicoimage/jpeg541667http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/4/Terpenes%20combinations%20inhibit%20biofilm%20formation%20in%20Staphyloccocus%20aureus%20by%20interfering%20with%20initial%20adhesion.jpg0d1d369962f1fa9d4a6cc2689baf0a92MD54TEXTTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion.pdf.txtTerpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion.pdf.txtExtracted texttext/plain50608http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4740/5/Terpenes%20combinations%20inhibit%20biofilm%20formation%20in%20Staphyloccocus%20aureus%20by%20interfering%20with%20initial%20adhesion.pdf.txt574810d792e2a3ac6f991b38bd812d80MD5520.500.14066/4740oai:repositorio.conacyt.gov.py:20.500.14066/47402026-04-28 13:58:09.159Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion Salinas Dávalos, Claudia de los Ángeles Biofilm S. aureus Terpenes |
| status_str | publishedVersion |
| title | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| title_full | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| title_fullStr | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| title_full_unstemmed | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| title_short | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| title_sort | Terpenes combinations inhibit biofilm formation in Staphyloccocus aureus by interfering with initial adhesion |
| topic | Biofilm S. aureus Terpenes |
| url | https://doi.org/10.3390/microorganisms10081527 http://hdl.handle.net/20.500.14066/4740 |