Screening of natural products inhibitors of SARS-CoV-2 entry
Correspondence: phsotelo@qui.una.py.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , , |
| Format: | article |
| Language: | English |
| Published: |
2022
|
| Subjects: | |
| Online Access: | https://doi.org/10.3390/molecules27051743 http://hdl.handle.net/20.500.14066/4591 |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1870612067814211584 |
|---|---|
| author | González Maldonado, Pamela |
| author2 | Alvarenga Sosa, Nelson Luis Burgos Edwards, Alberto Javier Flores Giubi, María Eugenia Barúa Chamorro, Javier Enrique Romero Rodríguez, María Cristina Soto Rifo, Ricardo Andrés Valiente Echeverría, Fernando Andrés Langjahr Penayo, Patricia Elena Cantero González, Guadalupe Luján Sotelo Torres, Pablo Hernán |
| author2_role | author author author author author author author author author author |
| author_browse | Alvarenga Sosa, Nelson Luis Barúa Chamorro, Javier Enrique Burgos Edwards, Alberto Javier Cantero González, Guadalupe Luján Flores Giubi, María Eugenia González Maldonado, Pamela Langjahr Penayo, Patricia Elena Romero Rodríguez, María Cristina Sotelo Torres, Pablo Hernán Soto Rifo, Ricardo Andrés Valiente Echeverría, Fernando Andrés |
| author_facet | González Maldonado, Pamela Alvarenga Sosa, Nelson Luis Burgos Edwards, Alberto Javier Flores Giubi, María Eugenia Barúa Chamorro, Javier Enrique Romero Rodríguez, María Cristina Soto Rifo, Ricardo Andrés Valiente Echeverría, Fernando Andrés Langjahr Penayo, Patricia Elena Cantero González, Guadalupe Luján Sotelo Torres, Pablo Hernán |
| author_role | author |
| bitstream.checksum.fl_str_mv | d7f771065032e0a4473209f7996df779 0175ea4a2d4caec4bbcc37e300941108 858b22fda432bd774e469302988c1974 5ecb84c6cd40fe21db60387369058a82 64cf6e767da280623fad9134d2a97f92 |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 MD5 MD5 MD5 |
| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/1/Screening%20of%20natural%20products%20inhibitors%20of%20SARS-CoV-2%20entry.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/4/Screening%20of%20natural%20products%20inhibitors%20of%20SARS-CoV-2%20entry.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/5/Screening%20of%20natural%20products%20inhibitors%20of%20SARS-CoV-2%20entry.pdf.txt |
| dc.contributor.other.es.fl_str_mv | Fundación Facultad de Ciencias Químicas |
| dc.creator.none.fl_str_mv | González Maldonado, Pamela Alvarenga Sosa, Nelson Luis Burgos Edwards, Alberto Javier Flores Giubi, María Eugenia Barúa Chamorro, Javier Enrique Romero Rodríguez, María Cristina Soto Rifo, Ricardo Andrés Valiente Echeverría, Fernando Andrés Langjahr Penayo, Patricia Elena Cantero González, Guadalupe Luján Sotelo Torres, Pablo Hernán |
| dc.date.accessioned.none.fl_str_mv | 2025-06-13T11:46:18Z |
| dc.date.available.none.fl_str_mv | 2025-06-13T11:46:18Z |
| dc.date.issued.none.fl_str_mv | 2022-03-07 |
| dc.format.extent.es.fl_str_mv | 10 páginas |
| dc.identifier.citation.en.fl_str_mv | González-Maldonado, P., Alvarenga, N., Burgos-Edwards, A., Flores-Giubi, M. E., Barúa, J. E., Romero-Rodríguez, M. C., Soto-Rifo, R., Valiente-Echeverría, F., Langjahr, P., Cantero-González, G., & Sotelo, P. H. (2022). Screening of natural products inhibitors of SARS-CoV-2 entry. Molecules, 27(5), Article 1743. https://doi.org/10.3390/molecules27051743 |
| dc.identifier.doi.es.fl_str_mv | 10.3390/molecules27051743 |
| dc.identifier.essn.es.fl_str_mv | 1420-3049 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.3390/molecules27051743 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4591 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| dc.relation.projectCONACYT.es.fl_str_mv | PINV20-6 BENA06-20 |
| dc.rights.*.fl_str_mv | Atribución 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.mesh.en.fl_str_mv | Oils, volatile Actinobacteria Antiviral agents HEK293 cells Angiotensin-converting enzyme 2 Plant extracts Spike glycoprotein, coronavirus Virus internalization Biological products SARS-CoV-2 COVID-19 drug treatment |
| dc.subject.mesh.es.fl_str_mv | Aceites volátiles Actinobacteria Antivirales Células HEK293 Enzima convertidora de angiotensina 2 Extractos vegetales Glicoproteína de la espiga del Coronavirus Internalización del virus Productos biológicos SARS-CoV-2 Tratamiento farmacológico de COVID-19 |
| dc.subject.other.es.fl_str_mv | Entry inhibitor Natural products Pseudotyped virus SARS-CoV-2 |
| dc.title.es.fl_str_mv | Screening of natural products inhibitors of SARS-CoV-2 entry |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Correspondence: phsotelo@qui.una.py. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_58e14933fcd0b5f7c0abaa09fd1bbefd |
| identifier_str_mv | González-Maldonado, P., Alvarenga, N., Burgos-Edwards, A., Flores-Giubi, M. E., Barúa, J. E., Romero-Rodríguez, M. C., Soto-Rifo, R., Valiente-Echeverría, F., Langjahr, P., Cantero-González, G., & Sotelo, P. H. (2022). Screening of natural products inhibitors of SARS-CoV-2 entry. Molecules, 27(5), Article 1743. https://doi.org/10.3390/molecules27051743 10.3390/molecules27051743 1420-3049 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4591 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| spelling | 15523000-3835-4a1e-8abb-4a22f1ef4079600466000000-0002-7949-7341851600444600134600101260022e2d875-b449-45ca-aebe-1c85545444186001c55d8f9-53ac-40e7-af9b-badbcadfcbf060064260047a9aee9-e928-4a75-be0e-080f7d304b4a60011006000000-0002-6473-2134Fundación Facultad de Ciencias Químicas2025-06-13T11:46:18Z2025-06-13T11:46:18Z2022-03-07González-Maldonado, P., Alvarenga, N., Burgos-Edwards, A., Flores-Giubi, M. E., Barúa, J. E., Romero-Rodríguez, M. C., Soto-Rifo, R., Valiente-Echeverría, F., Langjahr, P., Cantero-González, G., & Sotelo, P. H. (2022). Screening of natural products inhibitors of SARS-CoV-2 entry. Molecules, 27(5), Article 1743. https://doi.org/10.3390/molecules27051743https://doi.org/10.3390/molecules27051743http://hdl.handle.net/20.500.14066/459110.3390/molecules270517431420-3049Correspondence: phsotelo@qui.una.py.This article belongs to the Special Issue Improvements and Opportunities on Natural Products for Novel Drug Discovery.The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. The entry of the virus into the cell is one of the main targets for inhibiting SARS-CoV-2 infection. Natural products are an important source of new therapeutic alternatives against diseases. Pseudotyped viruses allow the study of SARS-CoV-2 viral entry inhibitors, and due to their simplicity, they allow the screening of a large number of antiviral candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 with the D614G SARS-CoV-2 spike glycoprotein to test its ability to infect ACE2-expressing HEK 293T cells in the presence of diverse natural products, including 21 plant extracts, 7 essential oils, and 13 compounds from plants and fungi. The 50% cytotoxic concentration (CC50) was evaluated using the resazurin method. From these analyses, we determined the inhibitory activity of the extract of Stachytarpheta cayennensis, which had a halfmaximal inhibitory concentration (IC50) of 91.65 _g/mL, a CC50 of 693.5 _g/mL, and a selectivity index (SI) of 7.57, indicating its potential use as an inhibitor of SARS-CoV-2 entry. Moreover, our work indicates the usefulness of the pseudotyped-virus system in the screening of SARS-CoV-2 entry inhibitors.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrolloPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Incentivos para la formación de investigadores en postgrados nacionales10 páginasengMultidisciplinary Digital Publishing InstituteAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).Aceites volátilesActinobacteriaAntiviralesCélulas HEK293Enzima convertidora de angiotensina 2Extractos vegetalesGlicoproteína de la espiga del CoronavirusInternalización del virusProductos biológicosSARS-CoV-2Tratamiento farmacológico de COVID-19Oils, volatileActinobacteriaAntiviral agentsHEK293 cellsAngiotensin-converting enzyme 2Plant extractsSpike glycoprotein, coronavirusVirus internalizationBiological productsSARS-CoV-2COVID-19 drug treatmentEntry inhibitorNatural productsPseudotyped virusSARS-CoV-2Screening of natural products inhibitors of SARS-CoV-2 entryinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion5MoleculesPINV20-6BENA06-2027González Maldonado, PamelaAlvarenga Sosa, Nelson LuisBurgos Edwards, Alberto JavierFlores Giubi, María EugeniaBarúa Chamorro, Javier EnriqueRomero Rodríguez, María CristinaSoto Rifo, Ricardo AndrésValiente Echeverría, Fernando AndrésLangjahr Penayo, Patricia ElenaCantero González, Guadalupe LujánSotelo Torres, Pablo HernánORIGINALScreening of natural products inhibitors of SARS-CoV-2 entry.pdfScreening of natural products inhibitors of SARS-CoV-2 entry.pdfArtículo científicoapplication/pdf1458706http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/1/Screening%20of%20natural%20products%20inhibitors%20of%20SARS-CoV-2%20entry.pdfd7f771065032e0a4473209f7996df779MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/2/license_rdf0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILScreening of natural products inhibitors of SARS-CoV-2 entry.jpgScreening of natural products inhibitors of SARS-CoV-2 entry.jpgVista de primera página de artículo científicoimage/jpeg974827http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/4/Screening%20of%20natural%20products%20inhibitors%20of%20SARS-CoV-2%20entry.jpg5ecb84c6cd40fe21db60387369058a82MD54TEXTScreening of natural products inhibitors of SARS-CoV-2 entry.pdf.txtScreening of natural products inhibitors of SARS-CoV-2 entry.pdf.txtExtracted texttext/plain39678http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4591/5/Screening%20of%20natural%20products%20inhibitors%20of%20SARS-CoV-2%20entry.pdf.txt64cf6e767da280623fad9134d2a97f92MD5520.500.14066/4591oai:repositorio.conacyt.gov.py:20.500.14066/45912026-02-12 19:30:41.867Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Screening of natural products inhibitors of SARS-CoV-2 entry González Maldonado, Pamela Aceites volátiles Actinobacteria Antivirales Células HEK293 Enzima convertidora de angiotensina 2 Extractos vegetales Glicoproteína de la espiga del Coronavirus Internalización del virus Productos biológicos SARS-CoV-2 Tratamiento farmacológico de COVID-19 Oils, volatile Actinobacteria Antiviral agents HEK293 cells Angiotensin-converting enzyme 2 Plant extracts Spike glycoprotein, coronavirus Virus internalization Biological products SARS-CoV-2 COVID-19 drug treatment Entry inhibitor Natural products Pseudotyped virus SARS-CoV-2 |
| status_str | publishedVersion |
| title | Screening of natural products inhibitors of SARS-CoV-2 entry |
| title_full | Screening of natural products inhibitors of SARS-CoV-2 entry |
| title_fullStr | Screening of natural products inhibitors of SARS-CoV-2 entry |
| title_full_unstemmed | Screening of natural products inhibitors of SARS-CoV-2 entry |
| title_short | Screening of natural products inhibitors of SARS-CoV-2 entry |
| title_sort | Screening of natural products inhibitors of SARS-CoV-2 entry |
| topic | Aceites volátiles Actinobacteria Antivirales Células HEK293 Enzima convertidora de angiotensina 2 Extractos vegetales Glicoproteína de la espiga del Coronavirus Internalización del virus Productos biológicos SARS-CoV-2 Tratamiento farmacológico de COVID-19 Oils, volatile Actinobacteria Antiviral agents HEK293 cells Angiotensin-converting enzyme 2 Plant extracts Spike glycoprotein, coronavirus Virus internalization Biological products SARS-CoV-2 COVID-19 drug treatment Entry inhibitor Natural products Pseudotyped virus SARS-CoV-2 |
| url | https://doi.org/10.3390/molecules27051743 http://hdl.handle.net/20.500.14066/4591 |