Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum
Corresponding author. Plataforma de Bioensaios de Malária e Leishmaniose (PBML), Fundação Oswaldo Cruz- Rondônia (FIOCRUZ-RO), Porto Velho, RO, Brazil. Corresponding author. Centro para el Desarrollo de la Investigación Científica (CEDIC), Asunción, Paraguay. E-mail addresses: jorwish@gmail.com (J.J...
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| Үндсэн зохиолч: | |
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| Бусад зохиолчид: | , , , , , , , , |
| Формат: | article |
| Хэл сонгох: | англи |
| Хэвлэсэн: |
2020
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| Нөхцлүүд: | |
| Онлайн хандалт: | https://doi.org/10.1016/j.toxcx.2020.100056 http://hdl.handle.net/20.500.14066/4594 |
| Шошгууд: |
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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| _version_ | 1870612068931993600 |
|---|---|
| author | Vitorino, Keila A. |
| author2 | Alfonso Ruiz Díaz, Jorge Javier Gómez Garay, Ana Fidelina Santos, Ana Paula A. Antunes, Ygor R. A. da S. Caldeira, Cleópatra Vega Gómez, María Celeste Teles, Carolina B.G. Soares, Andreimar M. Calderon, Leonardo A. |
| author2_role | author author author author author author author author author |
| author_browse | A. da S. Caldeira, Cleópatra Alfonso Ruiz Díaz, Jorge Javier Antunes, Ygor R. Calderon, Leonardo A. Gómez Garay, Ana Fidelina Santos, Ana Paula A. Soares, Andreimar M. Teles, Carolina B.G. Vega Gómez, María Celeste Vitorino, Keila A. |
| author_facet | Vitorino, Keila A. Alfonso Ruiz Díaz, Jorge Javier Gómez Garay, Ana Fidelina Santos, Ana Paula A. Antunes, Ygor R. A. da S. Caldeira, Cleópatra Vega Gómez, María Celeste Teles, Carolina B.G. Soares, Andreimar M. Calderon, Leonardo A. |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/1/Antimalarial%20activity%20of%20basic%20phospholipases%20A2%20isolated%20from%20Paraguayan%20Bothrops%20diporus%20venom%20against%20Plasmodium%20falciparum.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/4/Antimalarial%20activity%20of%20basic%20phospholipases%20A2%20isolated%20from%20Paraguayan%20Bothrops%20diporus%20venom%20against%20Plasmodium%20falciparum.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/5/Antimalarial%20activity%20of%20basic%20phospholipases%20A2%20isolated%20from%20Paraguayan%20Bothrops%20diporus%20venom%20against%20Plasmodium%20falciparum.pdf.txt |
| dc.creator.none.fl_str_mv | Vitorino, Keila A. Alfonso Ruiz Díaz, Jorge Javier Gómez Garay, Ana Fidelina Santos, Ana Paula A. Antunes, Ygor R. A. da S. Caldeira, Cleópatra Vega Gómez, María Celeste Teles, Carolina B.G. Soares, Andreimar M. Calderon, Leonardo A. |
| dc.date.accessioned.none.fl_str_mv | 2025-06-18T14:49:09Z |
| dc.date.available.none.fl_str_mv | 2025-06-18T14:49:09Z |
| dc.date.issued.none.fl_str_mv | 2020-08-08 |
| dc.format.extent.es.fl_str_mv | 8 páginas |
| dc.identifier.citation.en.fl_str_mv | Vitorino, K. A., Alfonso, J. J., Gómez, A. F., Santos, A. P. A., Antunes, Y. R., Caldeira, C. A. S., Gómez, C. V., Teles, C. B. G., Soares, A. M., & Calderon, L. A. (2020). Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum. Toxicon: X, 8, Article 100056. https://doi.org/10.1016/j.toxcx.2020.100056 |
| dc.identifier.doi.es.fl_str_mv | 10.1016/j.toxcx.2020.100056 |
| dc.identifier.essn.es.fl_str_mv | 2590-1710 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.1016/j.toxcx.2020.100056 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4594 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Elsevier |
| dc.rights.*.fl_str_mv | Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.subject.other.es.fl_str_mv | Antiparasitic activity Bothrops diporus Phospholipases A(2) Plasmodium falciparum Snake venom |
| dc.title.es.fl_str_mv | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Corresponding author. Plataforma de Bioensaios de Malária e Leishmaniose (PBML), Fundação Oswaldo Cruz- Rondônia (FIOCRUZ-RO), Porto Velho, RO, Brazil. Corresponding author. Centro para el Desarrollo de la Investigación Científica (CEDIC), Asunción, Paraguay. E-mail addresses: jorwish@gmail.com (J.J. Alfonso), paulaazevedo.2011@gmail.com (A.P.A. Santos). |
| eu_rights_str_mv | openAccess |
| format | article |
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| identifier_str_mv | Vitorino, K. A., Alfonso, J. J., Gómez, A. F., Santos, A. P. A., Antunes, Y. R., Caldeira, C. A. S., Gómez, C. V., Teles, C. B. G., Soares, A. M., & Calderon, L. A. (2020). Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum. Toxicon: X, 8, Article 100056. https://doi.org/10.1016/j.toxcx.2020.100056 10.1016/j.toxcx.2020.100056 2590-1710 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4594 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | b0b2d07e-3e9e-43a9-b014-2ef12ca043596003660052460049ab5fde-6592-4278-8ce1-2cb954daed9b6003a0b6de7-9e11-40dd-8327-39271741451d600b071db49-18c6-4709-a1ad-b18f1c79311160011456000000-0001-6291-431624876298-0ad5-493b-b452-d1368c946017600565e86b4-935a-4b97-9c01-52f51ab8cc0e60018204809-987b-4442-8250-b4dec6a10a866002025-06-18T14:49:09Z2025-06-18T14:49:09Z2020-08-08Vitorino, K. A., Alfonso, J. J., Gómez, A. F., Santos, A. P. A., Antunes, Y. R., Caldeira, C. A. S., Gómez, C. V., Teles, C. B. G., Soares, A. M., & Calderon, L. A. (2020). Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum. Toxicon: X, 8, Article 100056. https://doi.org/10.1016/j.toxcx.2020.100056https://doi.org/10.1016/j.toxcx.2020.100056http://hdl.handle.net/20.500.14066/459410.1016/j.toxcx.2020.1000562590-1710Corresponding author. Plataforma de Bioensaios de Malária e Leishmaniose (PBML), Fundação Oswaldo Cruz- Rondônia (FIOCRUZ-RO), Porto Velho, RO, Brazil. Corresponding author. Centro para el Desarrollo de la Investigación Científica (CEDIC), Asunción, Paraguay. E-mail addresses: jorwish@gmail.com (J.J. Alfonso), paulaazevedo.2011@gmail.com (A.P.A. Santos).Malaria is a parasitic infectious disease and was responsible for 400.000 deaths in 2018. Plasmodium falciparum represents the species that causes most human deaths due to severe malaria. In addition, studies prove the resistance of P. falciparum to drugs used to treat malaria, making the search for new drugs with antiplasmodial potential necessary. In this context, the literature describes snake venoms as a rich source of molecules with microbicidal potential, including phospholipases A2 (PLA2s). In this sense, the present study aimed to isolate basic PLA2s from Paraguayan Bothrops diporus venom and evaluate their antiplasmodial potential. Basic PLA2s were obtained using two chromatographic steps. Initially, B. diporus venom was subjected to ion exchange chromatography (IEC). The electrophoretic profile of the fractions from the IEC permitted the selection of 3 basic fractions, which were subjected to reverse phase chromatography, resulting in the isolation of the PLA2s. The toxins were tested for enzymatic activity using a chromogenic substrate and finally, the antiplasmodial, cytotoxic potential and hemolytic activity of the isolated toxins were evaluated. The electrophoretic profile of the fractions from the IEC permitted the selection of 3 basic fractions, which were subjected to reverse phase chromatography, resulting in the isolation of the two enzymatically active PLA2s, BdTX-I and BdTX-II and the PLA2 homologue BdTX-III. The antiplasmodial potential was evaluated and the toxins showed IC50 values of: 2.44, 0.0153 and 0.59 μg/mL respectively, presenting PLA2 selectivity according to the selectivity index results (SI) calculated against HepG2 cells. The results show that the 3 basic phospholipases isolated in this study have a potent antiparasitic effect against the W2 strain of P. falciparum. In view of the results obtained in this work, further research are necessary to determine the mechanism of action by which these toxins cause cell death in parasites.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Programa Nacional de Incentivo a los Investigadores8 páginasengElsevierAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccess© 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Antiparasitic activityBothrops diporusPhospholipases A(2)Plasmodium falciparumSnake venomAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparuminfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionToxicon: X8Vitorino, Keila A.Alfonso Ruiz Díaz, Jorge JavierGómez Garay, Ana FidelinaSantos, Ana Paula A.Antunes, Ygor R.A. da S. Caldeira, CleópatraVega Gómez, María CelesteTeles, Carolina B.G.Soares, Andreimar M.Calderon, Leonardo A.ORIGINALAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum.pdfAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum.pdfArtículo científicoapplication/pdf1566706http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/1/Antimalarial%20activity%20of%20basic%20phospholipases%20A2%20isolated%20from%20Paraguayan%20Bothrops%20diporus%20venom%20against%20Plasmodium%20falciparum.pdfdf9132b96882250f468a4e8fe7f125c8MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum.jpgAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum.jpgVista de primera página de artículo científicoimage/jpeg922072http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/4/Antimalarial%20activity%20of%20basic%20phospholipases%20A2%20isolated%20from%20Paraguayan%20Bothrops%20diporus%20venom%20against%20Plasmodium%20falciparum.jpgf29619f4550dc797b271fc6c0a149046MD54TEXTAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum.pdf.txtAntimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum.pdf.txtExtracted texttext/plain52084http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4594/5/Antimalarial%20activity%20of%20basic%20phospholipases%20A2%20isolated%20from%20Paraguayan%20Bothrops%20diporus%20venom%20against%20Plasmodium%20falciparum.pdf.txt416d387cd5c60fdf912664240a4992f6MD5520.500.14066/4594oai:repositorio.conacyt.gov.py:20.500.14066/45942026-02-12 19:30:42.639Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum Vitorino, Keila A. Antiparasitic activity Bothrops diporus Phospholipases A(2) Plasmodium falciparum Snake venom |
| status_str | publishedVersion |
| title | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| title_full | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| title_fullStr | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| title_full_unstemmed | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| title_short | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| title_sort | Antimalarial activity of basic phospholipases A2 isolated from Paraguayan Bothrops diporus venom against Plasmodium falciparum |
| topic | Antiparasitic activity Bothrops diporus Phospholipases A(2) Plasmodium falciparum Snake venom |
| url | https://doi.org/10.1016/j.toxcx.2020.100056 http://hdl.handle.net/20.500.14066/4594 |