Characterization of dengue virus 4 cases in Paraguay, 2019–2020
Correspondence: arojass@iics.una.py (A.R.); jjwaggo@emory.edu (J.J.W.)
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| Format: | article |
| Language: | English |
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2024
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| Online Access: | http://hdl.handle.net/20.500.14066/4367 |
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| _version_ | 1870612069874663424 |
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| author | Rojas Segovia, Alejandra María |
| author2 | Shen, John Cardozo, Fátima Bernal, Cynthia Caballero, Oliver Ping, Sara Key, Autum Haider, Ali Arévalo de Guillén, Yvalena Langjahr Penayo, Patricia Elena Acosta de Hetter, María Eugenia Aria Zaya, Laura Silvana Mendoza Torres, Laura Patricia Páez Acchiardi, Gloria Malvina Von-Horoch, Marta Luraschi, Patricia Cabral, Sandra Sánchez, María Cecilia Torres, Aurelia Pinsky, Benjamin A. Piantadosi, Anne Waggoner, Jesse J. |
| author2_role | author author author author author author author author author author author author author author author author author author author author author |
| author_browse | Acosta de Hetter, María Eugenia Aria Zaya, Laura Silvana Arévalo de Guillén, Yvalena Bernal, Cynthia Caballero, Oliver Cabral, Sandra Cardozo, Fátima Haider, Ali Key, Autum Langjahr Penayo, Patricia Elena Luraschi, Patricia Mendoza Torres, Laura Patricia Piantadosi, Anne Ping, Sara Pinsky, Benjamin A. Páez Acchiardi, Gloria Malvina Rojas Segovia, Alejandra María Shen, John Sánchez, María Cecilia Torres, Aurelia Von-Horoch, Marta Waggoner, Jesse J. |
| author_facet | Rojas Segovia, Alejandra María Shen, John Cardozo, Fátima Bernal, Cynthia Caballero, Oliver Ping, Sara Key, Autum Haider, Ali Arévalo de Guillén, Yvalena Langjahr Penayo, Patricia Elena Acosta de Hetter, María Eugenia Aria Zaya, Laura Silvana Mendoza Torres, Laura Patricia Páez Acchiardi, Gloria Malvina Von-Horoch, Marta Luraschi, Patricia Cabral, Sandra Sánchez, María Cecilia Torres, Aurelia Pinsky, Benjamin A. Piantadosi, Anne Waggoner, Jesse J. |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/1/Characterization%20of%20Dengue%20Virus%204%20Cases%20in%20Paraguay%2c%202019%e2%80%932020.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/4/Characterization%20of%20Dengue%20Virus%204%20Cases%20in%20Paraguay%2c%202019%e2%80%932020%20SM.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/5/Characterization%20of%20Dengue%20Virus%204%20Cases%20in%20Paraguay%2c%202019%e2%80%932020.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/6/Characterization%20of%20Dengue%20Virus%204%20Cases%20in%20Paraguay%2c%202019%e2%80%932020.pdf.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/7/Characterization%20of%20Dengue%20Virus%204%20Cases%20in%20Paraguay%2c%202019%e2%80%932020%20SM.pdf.txt |
| dc.contributor.other.es.fl_str_mv | Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud |
| dc.creator.none.fl_str_mv | Rojas Segovia, Alejandra María Shen, John Cardozo, Fátima Bernal, Cynthia Caballero, Oliver Ping, Sara Key, Autum Haider, Ali Arévalo de Guillén, Yvalena Langjahr Penayo, Patricia Elena Acosta de Hetter, María Eugenia Aria Zaya, Laura Silvana Mendoza Torres, Laura Patricia Páez Acchiardi, Gloria Malvina Von-Horoch, Marta Luraschi, Patricia Cabral, Sandra Sánchez, María Cecilia Torres, Aurelia Pinsky, Benjamin A. Piantadosi, Anne Waggoner, Jesse J. |
| dc.date.accessioned.none.fl_str_mv | 2024-02-08T14:20:55Z |
| dc.date.available.none.fl_str_mv | 2024-02-08T14:20:55Z |
| dc.date.issued.none.fl_str_mv | 2024-01-25 |
| dc.identifier.citation.en.fl_str_mv | Rojas, A.; Shen, J.; Cardozo, F.; Bernal, C.; Caballero, O.; Ping, S.; Key, A.; Haider, A.; de Guillen, Y.; Langjahr, P.; et al. Characterization of dengue virus 4 cases in Paraguay, 2019–2020. Viruses 2024, 16, 181. https://doi.org/10.3390/v16020181 |
| dc.identifier.doi.es.fl_str_mv | 10.3390/v16020181 |
| dc.identifier.essn.es.fl_str_mv | 1999-4915 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4367 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| dc.relation.projectCONACYT.es.fl_str_mv | PINV18-1295 |
| dc.rights.*.fl_str_mv | Atribución 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.copyright.es.fl_str_mv | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.classification.es.fl_str_mv | 7. Salud 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles |
| dc.subject.ocde.es.fl_str_mv | 3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología) |
| dc.subject.other.es.fl_str_mv | Dengue NS1 Paraguay Phylogenetic analysis rRT-PCR |
| dc.title.es.fl_str_mv | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Correspondence: arojass@iics.una.py (A.R.); jjwaggo@emory.edu (J.J.W.) |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_93df56bbc67e6878aaccc877bfc3d7d8 |
| identifier_str_mv | Rojas, A.; Shen, J.; Cardozo, F.; Bernal, C.; Caballero, O.; Ping, S.; Key, A.; Haider, A.; de Guillen, Y.; Langjahr, P.; et al. Characterization of dengue virus 4 cases in Paraguay, 2019–2020. Viruses 2024, 16, 181. https://doi.org/10.3390/v16020181 10.3390/v16020181 1999-4915 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4367 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ © 2024 by the authors. Licensee MDPI, Basel, Switzerland. |
| spelling | 10026002e9ae439-3692-4719-82c9-7eaa01ee609060041091ed8-8aaa-4f31-bf3b-b99241ab14cd600142c16ce-dd78-4237-b975-aaa64a424ff96004c48e764-7a69-4502-a617-70faa13d797e600615deef7-0b5d-4074-bcf8-4dfb7998687e6001c74c2c8-19bc-4df0-bf20-4d91a99c142a600c973c5da-2abe-4dbf-b027-805c75e2e7016009bf1082e-0b69-4c45-988c-6633da9d1b73600642600146000000-0003-0801-4397826000000-0002-5442-12017426000000-0001-6124-124485660074db3d25-d930-4095-b5c0-65bf3c2669866006ff9408f-2e2a-481b-aedf-d64e8df85e526008ca04f0c-0c0d-4759-9182-45e846fdc794600ca0d95cf-1f73-445f-9b2f-dfccfb24dd92600de66270e-a87c-4a57-af2e-d51741dc51e7600bb370a93-63ed-4720-a616-649a6874ed2b600204973db-e5f6-4ca6-9f37-d214dac166186002d504966-0a96-4d8c-8e00-c59a6d0a0609600Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud2024-02-08T14:20:55Z2024-02-08T14:20:55Z2024-01-25Rojas, A.; Shen, J.; Cardozo, F.; Bernal, C.; Caballero, O.; Ping, S.; Key, A.; Haider, A.; de Guillen, Y.; Langjahr, P.; et al. Characterization of dengue virus 4 cases in Paraguay, 2019–2020. Viruses 2024, 16, 181. https://doi.org/10.3390/v16020181http://hdl.handle.net/20.500.14066/436710.3390/v160201811999-4915Correspondence: arojass@iics.una.py (A.R.); jjwaggo@emory.edu (J.J.W.)In 2019–2020, dengue virus (DENV) type 4 emerged to cause the largest DENV outbreak in Paraguay’s history. This study sought to characterize dengue relative to other acute illness cases and use phylogenetic analysis to understand the outbreak’s origin. Individuals with an acute illness (≤7 days) were enrolled and tested for DENV nonstructural protein 1 (NS1) and viral RNA by real-time RT-PCR. Near-complete genome sequences were obtained from 62 DENV-4 positive samples. From January 2019 to March 2020, 799 participants were enrolled: 253 dengue (14 severe dengue, 5.5%) and 546 other acute illness cases. DENV-4 was detected in 238 dengue cases (94.1%). NS1 detection by rapid test was 52.5% sensitive (53/101) and 96.5% specific (387/401) for dengue compared to rRT-PCR. DENV-4 sequences were grouped into two clades within genotype II. No clustering was observed based on dengue severity, location, or date. Sequences obtained here were most closely related to 2018 DENV-4 sequences from Paraguay, followed by a 2013 sequence from southern Brazil. DENV-4 can result in large outbreaks, including severe cases, and is poorly detected with available rapid diagnostics. Outbreak strains seem to have been circulating in Paraguay and Brazil prior to 2018, highlighting the importance of sustained DENV genomic surveillance.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrolloengMultidisciplinary Digital Publishing InstituteAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2024 by the authors. Licensee MDPI, Basel, Switzerland.7. Salud7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisiblesDengueNS1ParaguayPhylogenetic analysisrRT-PCR3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología)Characterization of dengue virus 4 cases in Paraguay, 2019–2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion2VirusesPINV18-129516Rojas Segovia, Alejandra MaríaShen, JohnCardozo, FátimaBernal, CynthiaCaballero, OliverPing, SaraKey, AutumHaider, AliArévalo de Guillén, YvalenaLangjahr Penayo, Patricia ElenaAcosta de Hetter, María EugeniaAria Zaya, Laura SilvanaMendoza Torres, Laura PatriciaPáez Acchiardi, Gloria MalvinaVon-Horoch, MartaLuraschi, PatriciaCabral, SandraSánchez, María CeciliaTorres, AureliaPinsky, Benjamin A.Piantadosi, AnneWaggoner, Jesse J.ORIGINALCharacterization of Dengue Virus 4 Cases in Paraguay, 2019–2020.pdfCharacterization of Dengue Virus 4 Cases in Paraguay, 2019–2020.pdfArtículo científicoapplication/pdf3138428http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4367/1/Characterization%20of%20Dengue%20Virus%204%20Cases%20in%20Paraguay%2c%202019%e2%80%932020.pdf4f6ac01add0d2948aae76d3f07f604cdMD51Characterization of Dengue Virus 4 Cases in Paraguay, 2019–2020 SM.pdfCharacterization of Dengue Virus 4 Cases in Paraguay, 2019–2020 SM.pdfAnexo I. 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| spellingShingle | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 Rojas Segovia, Alejandra María 7. Salud 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles Dengue NS1 Paraguay Phylogenetic analysis rRT-PCR 3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología) |
| status_str | publishedVersion |
| title | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| title_full | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| title_fullStr | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| title_full_unstemmed | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| title_short | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| title_sort | Characterization of dengue virus 4 cases in Paraguay, 2019–2020 |
| topic | 7. Salud 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles Dengue NS1 Paraguay Phylogenetic analysis rRT-PCR 3.3. Ciencias de la salud (salud pública, medicina social, higiene, enfermería, epidemiología) |
| url | http://hdl.handle.net/20.500.14066/4367 |