Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay
Correspondence: gloresqx@agr.una.py (G.R.-R.); regina.carneiro@embrapa.br (R.M.D.G.C.); Tel.: +595-21-585606/10 (G.R.-R.); +55-(61)-3448-4433 (R.M.D.G.C.); Fax: +595-21-585613 (G.R.-R.); +55-(61)-3448-4890 or +55-(61)-3448-4891 (R.M.D.G.C.).
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| מחברים אחרים: | , , , , , , |
| פורמט: | article |
| שפה: | אנגלית |
| יצא לאור: |
2023
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| נושאים: | |
| גישה מקוונת: | https://doi.org/10.3390/agronomy13030670 http://hdl.handle.net/20.500.14066/4726 |
| תגים: |
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| _version_ | 1870612071862763520 |
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| author | Resquín Romero, Gloria Arminda |
| author2 | Silva Mattos, Vanessa da Mata dos Santos Monteiro, Jessica López Nicora, Horacio Daniel Amarilla, Shyrley Paola Chamorro Díaz, Sergio Manuel Moral Moral, Juan Dechechi Gomes Carneiro, Regina María |
| author2_role | author author author author author author author |
| author_browse | Amarilla, Shyrley Paola Chamorro Díaz, Sergio Manuel Dechechi Gomes Carneiro, Regina María López Nicora, Horacio Daniel Moral Moral, Juan Resquín Romero, Gloria Arminda Silva Mattos, Vanessa da Mata dos Santos Monteiro, Jessica |
| author_facet | Resquín Romero, Gloria Arminda Silva Mattos, Vanessa da Mata dos Santos Monteiro, Jessica López Nicora, Horacio Daniel Amarilla, Shyrley Paola Chamorro Díaz, Sergio Manuel Moral Moral, Juan Dechechi Gomes Carneiro, Regina María |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/4/Enzymatic%20and%20molecular%20identification%20of%20Meloidogyne%20species%20in%20tomato%20orchards%20in%20Paraguay.jpg http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/1/Enzymatic%20and%20molecular%20identification%20of%20Meloidogyne%20species%20in%20tomato%20orchards%20in%20Paraguay.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/2/license_rdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/3/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/5/Enzymatic%20and%20molecular%20identification%20of%20Meloidogyne%20species%20in%20tomato%20orchards%20in%20Paraguay.pdf.txt |
| dc.creator.none.fl_str_mv | Resquín Romero, Gloria Arminda Silva Mattos, Vanessa da Mata dos Santos Monteiro, Jessica López Nicora, Horacio Daniel Amarilla, Shyrley Paola Chamorro Díaz, Sergio Manuel Moral Moral, Juan Dechechi Gomes Carneiro, Regina María |
| dc.date.accessioned.none.fl_str_mv | 2025-12-30T17:44:59Z |
| dc.date.available.none.fl_str_mv | 2025-12-30T17:44:59Z |
| dc.date.issued.none.fl_str_mv | 2023-02-25 |
| dc.format.extent.es.fl_str_mv | 10 páginas |
| dc.identifier.citation.en.fl_str_mv | Resquín-Romero, G., Mattos, V. S., Monteiro, J. M. S., Lopez-Nicora, H. D., Amarilla, S. P., Chamorro-Diaz, S., Moral, J., & Carneiro, R. M. D. G. (2023). Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay. Agronomy, 13(3), Article 670. https://doi.org/10.3390/agronomy13030670 |
| dc.identifier.doi.es.fl_str_mv | 10.3390/agronomy13030670 |
| dc.identifier.issn.es.fl_str_mv | 2073-4395 |
| dc.identifier.other.es.fl_str_mv | https://doi.org/10.3390/agronomy13030670 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/4726 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Multidisciplinary Digital Publishing Institute |
| dc.rights.*.fl_str_mv | Atribución/Reconocimiento 4.0 Internacional |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.uri.*.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.subject.other.es.fl_str_mv | Esterase phenotype Meloidogyne incognita Meloidogyne javanica Root-knot nematode SCAR markers |
| dc.title.es.fl_str_mv | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Correspondence: gloresqx@agr.una.py (G.R.-R.); regina.carneiro@embrapa.br (R.M.D.G.C.); Tel.: +595-21-585606/10 (G.R.-R.); +55-(61)-3448-4433 (R.M.D.G.C.); Fax: +595-21-585613 (G.R.-R.); +55-(61)-3448-4890 or +55-(61)-3448-4891 (R.M.D.G.C.). |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_c68406e281e153aaeabd152cdf12a033 |
| identifier_str_mv | Resquín-Romero, G., Mattos, V. S., Monteiro, J. M. S., Lopez-Nicora, H. D., Amarilla, S. P., Chamorro-Diaz, S., Moral, J., & Carneiro, R. M. D. G. (2023). Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay. Agronomy, 13(3), Article 670. https://doi.org/10.3390/agronomy13030670 2073-4395 10.3390/agronomy13030670 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/4726 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | Atribución/Reconocimiento 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ |
| spelling | 944600a56ee728-2141-4d2e-bc20-84a5f3dfb425600625ed853-07db-45cb-9e79-f29d9281070b6006806000000-0002-9672-9589546007172cff1-f98f-4c95-b719-212b5d524ca0600f6238b4a-9c3c-43fc-90a8-4198a09c2f3460068a70460-40a7-4bf7-add6-792988e67dd16002025-12-30T17:44:59Z2025-12-30T17:44:59Z2023-02-25Resquín-Romero, G., Mattos, V. S., Monteiro, J. M. S., Lopez-Nicora, H. D., Amarilla, S. P., Chamorro-Diaz, S., Moral, J., & Carneiro, R. M. D. G. (2023). Enzymatic and Molecular Identification of Meloidogyne Species in Tomato Orchards in Paraguay. Agronomy, 13(3), Article 670. https://doi.org/10.3390/agronomy130306702073-4395https://doi.org/10.3390/agronomy13030670http://hdl.handle.net/20.500.14066/472610.3390/agronomy13030670Correspondence: gloresqx@agr.una.py (G.R.-R.); regina.carneiro@embrapa.br (R.M.D.G.C.); Tel.: +595-21-585606/10 (G.R.-R.); +55-(61)-3448-4433 (R.M.D.G.C.); Fax: +595-21-585613 (G.R.-R.); +55-(61)-3448-4890 or +55-(61)-3448-4891 (R.M.D.G.C.).This article belongs to the Section Pest and Disease Management.Tomato is a major crop in Paraguay, where it provides a source of employment and income for households. Tomato production can be affected by root-knot nematodes, especially Meloidogyne spp. The unequivocal identification of Meloidogyne spp. in Paraguay has not been conducted yet. This study aims to identify Meloidogyne species in eight tomato production districts of this country by biochemical and molecular techniques. Females of Meloidogyne spp. were extracted from tomato roots and characterized using esterase isozyme phenotypes. In addition, DNA was extracted from nematode eggs, and species-specific SCARs (sequence-characterized amplified regions) were used to confirm the diagnosis. Nematodes were detected in 100% of studied samples (prevalence), of which M. incognita (Est: I2, Rm: 1.1;1.2) and M. javanica (Est: J3, Rm: 1.0, 1.20, 1.35) were present in 39.13% and 26.08% of samples, respectively. One population (8.69%) of Meloidogyne sp. presenting an atypical esterase profile (Rm: 1.0 and 1.3) was only detected in Julián Augusto Saldívar District. Mixed populations, mostly M. incognita and M. javanica, were observed in 26.08% of samples. The SCAR primers incK14F/incK14R amplified specific fragments for M. incognita (399 bp) and M. javanica (670 bp), confirming the enzymatic results. Here, we present the first study of root-knot nematode identification at the species level in Paraguay.Consejo Nacional de Ciencia y Tecnología10 páginasengMultidisciplinary Digital Publishing InstituteAtribución/Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/).Esterase phenotypeMeloidogyne incognitaMeloidogyne javanicaRoot-knot nematodeSCAR markersEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguayinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion3Agronomy13Resquín Romero, Gloria ArmindaSilva Mattos, Vanessada Mata dos Santos Monteiro, JessicaLópez Nicora, Horacio DanielAmarilla, Shyrley PaolaChamorro Díaz, Sergio ManuelMoral Moral, JuanDechechi Gomes Carneiro, Regina MaríaTHUMBNAILEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay.jpgEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay.jpgArtículo científicoimage/jpeg1056589http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/4/Enzymatic%20and%20molecular%20identification%20of%20Meloidogyne%20species%20in%20tomato%20orchards%20in%20Paraguay.jpg1009a591d5a824d93fd816af33e1ec83MD54ORIGINALEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay.pdfEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay.pdfArtículo científicoapplication/pdf14524327http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/1/Enzymatic%20and%20molecular%20identification%20of%20Meloidogyne%20species%20in%20tomato%20orchards%20in%20Paraguay.pdf97042ff923831592bf753bc8079384e5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/2/license_rdf0175ea4a2d4caec4bbcc37e300941108MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/3/license.txt858b22fda432bd774e469302988c1974MD53TEXTEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay.pdf.txtEnzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay.pdf.txtExtracted texttext/plain39222http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4726/5/Enzymatic%20and%20molecular%20identification%20of%20Meloidogyne%20species%20in%20tomato%20orchards%20in%20Paraguay.pdf.txt210b99380ce19a70141dc439c966432dMD5520.500.14066/4726oai:repositorio.conacyt.gov.py:20.500.14066/47262025-12-31 02:17:28.52Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay Resquín Romero, Gloria Arminda Esterase phenotype Meloidogyne incognita Meloidogyne javanica Root-knot nematode SCAR markers |
| status_str | publishedVersion |
| title | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| title_full | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| title_fullStr | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| title_full_unstemmed | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| title_short | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| title_sort | Enzymatic and molecular identification of Meloidogyne species in tomato orchards in Paraguay |
| topic | Esterase phenotype Meloidogyne incognita Meloidogyne javanica Root-knot nematode SCAR markers |
| url | https://doi.org/10.3390/agronomy13030670 http://hdl.handle.net/20.500.14066/4726 |