Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking

Correspondence: nverabilbao@gmail.com (N.V.d.B.); alain.fournet@orange.fr (A.F.)

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Main Author: Vera de Bilbao, Ninfa (author)
Other Authors: Giebelhaus, Ryland T. (author), Dias, Ryan P. (author), Ferreira de Paredes, María Elena (author), Martínez, Miguel (author), Velasco Carneros, Lorea (author), Nam, Seo Lin (author), de la Mata, Aidee Paulina (author), Maréchal, Jean-Didier (author), Adou, Ahissan Innocent (author), Yaluff Miranda, Gloria Cristina (author), Serna, María Elva (author), Sylvestre, Muriel (author), Torres de Ortiz, Agueda Susana (author), Schinini Vega, Alicia Petrona (author), Galeano, Ricardo (author), Fournet, Alain (author), Harynuk, James J. (author), Cebrian Torrejon, Gerardo (author)
Format: article
Language:English
Published: 2025
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Online Access:https://doi.org/10.3390/plants14060954
http://hdl.handle.net/20.500.14066/4552
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author Vera de Bilbao, Ninfa
author2 Giebelhaus, Ryland T.
Dias, Ryan P.
Ferreira de Paredes, María Elena
Martínez, Miguel
Velasco Carneros, Lorea
Nam, Seo Lin
de la Mata, Aidee Paulina
Maréchal, Jean-Didier
Adou, Ahissan Innocent
Yaluff Miranda, Gloria Cristina
Serna, María Elva
Sylvestre, Muriel
Torres de Ortiz, Agueda Susana
Schinini Vega, Alicia Petrona
Galeano, Ricardo
Fournet, Alain
Harynuk, James J.
Cebrian Torrejon, Gerardo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author_browse Adou, Ahissan Innocent
Cebrian Torrejon, Gerardo
Dias, Ryan P.
Ferreira de Paredes, María Elena
Fournet, Alain
Galeano, Ricardo
Giebelhaus, Ryland T.
Harynuk, James J.
Martínez, Miguel
Maréchal, Jean-Didier
Nam, Seo Lin
Schinini Vega, Alicia Petrona
Serna, María Elva
Sylvestre, Muriel
Torres de Ortiz, Agueda Susana
Velasco Carneros, Lorea
Vera de Bilbao, Ninfa
Yaluff Miranda, Gloria Cristina
de la Mata, Aidee Paulina
author_facet Vera de Bilbao, Ninfa
Giebelhaus, Ryland T.
Dias, Ryan P.
Ferreira de Paredes, María Elena
Martínez, Miguel
Velasco Carneros, Lorea
Nam, Seo Lin
de la Mata, Aidee Paulina
Maréchal, Jean-Didier
Adou, Ahissan Innocent
Yaluff Miranda, Gloria Cristina
Serna, María Elva
Sylvestre, Muriel
Torres de Ortiz, Agueda Susana
Schinini Vega, Alicia Petrona
Galeano, Ricardo
Fournet, Alain
Harynuk, James J.
Cebrian Torrejon, Gerardo
author_role author
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dc.contributor.other.es.fl_str_mv Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud
dc.creator.none.fl_str_mv Vera de Bilbao, Ninfa
Giebelhaus, Ryland T.
Dias, Ryan P.
Ferreira de Paredes, María Elena
Martínez, Miguel
Velasco Carneros, Lorea
Nam, Seo Lin
de la Mata, Aidee Paulina
Maréchal, Jean-Didier
Adou, Ahissan Innocent
Yaluff Miranda, Gloria Cristina
Serna, María Elva
Sylvestre, Muriel
Torres de Ortiz, Agueda Susana
Schinini Vega, Alicia Petrona
Galeano, Ricardo
Fournet, Alain
Harynuk, James J.
Cebrian Torrejon, Gerardo
dc.date.accessioned.none.fl_str_mv 2025-04-02T17:31:30Z
dc.date.available.none.fl_str_mv 2025-04-02T17:31:30Z
dc.date.issued.none.fl_str_mv 2025-03-18
dc.identifier.citation.en.fl_str_mv de Bilbao, N. V., Giebelhaus, R. T., Dias, R. P., Ferreira, M. E., Martínez, M., Velasco Carneros, L., Nam, S. L., de la Mata, A. P., Maréchal, J. D., Adou, A. I., Yaluff, G., Serna, E., Sylvestre, M., Torres, S., Schinini, A., Galeano, R., Fournet, A., Harynuk, J. J., & Cebrián Torrejón, G. (2025). Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking. Plants, 14(6), Artículo 954. https://doi.org/10.3390/plants14060954
dc.identifier.doi.es.fl_str_mv 10.3390/plants14060954
dc.identifier.essn.es.fl_str_mv 2223-7747
dc.identifier.other.es.fl_str_mv https://doi.org/10.3390/plants14060954
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.14066/4552
dc.language.iso.es.fl_str_mv eng
dc.publisher.es.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.relation.projectCONACYT.es.fl_str_mv 14-INV-151
dc.rights.*.fl_str_mv Atribución 4.0 Internacional
dc.rights.accessRights.es.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.subject.classification.en.fl_str_mv 6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations
dc.subject.classification.es.fl_str_mv 6. Producción y tecnología industrial
dc.subject.other.es.fl_str_mv Canthin-6-ones
Chagas disease
Medicinal plants
Natural products
Plant metabolomics
Rutaceae
dc.title.es.fl_str_mv Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
dc.type.es.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description Correspondence: nverabilbao@gmail.com (N.V.d.B.); alain.fournet@orange.fr (A.F.)
eu_rights_str_mv openAccess
format article
id CONACYT_da2fa066404b8373e084aa5efb47486a
identifier_str_mv de Bilbao, N. V., Giebelhaus, R. T., Dias, R. P., Ferreira, M. E., Martínez, M., Velasco Carneros, L., Nam, S. L., de la Mata, A. P., Maréchal, J. D., Adou, A. I., Yaluff, G., Serna, E., Sylvestre, M., Torres, S., Schinini, A., Galeano, R., Fournet, A., Harynuk, J. J., & Cebrián Torrejón, G. (2025). Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking. Plants, 14(6), Artículo 954. https://doi.org/10.3390/plants14060954
10.3390/plants14060954
2223-7747
language eng
network_acronym_str CONACYT
network_name_str Repositorio Institucional CONACYT
oai_identifier_str oai:repositorio.conacyt.gov.py:20.500.14066/4552
publishDate 2025
publishDateSort 2025
repository.mail.fl_str_mv repositorio.institucional@conacyt.gov.py
repository.name.fl_str_mv Repositorio Institucional CONACYT
repository_id_str
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
spelling a5f42a64-d842-4f2f-839a-f0860194e72d6003bbc804d-1699-428d-a70b-18653bf6a999600323fdbde-718f-42f2-85c0-d35fd6bcc3bf6004296007146008f91841d-3c31-42e2-9f26-2477611979b9600e751cd2b-8803-4aa1-ab5a-8488e248b496600c650b955-5330-4b84-90da-442485acecb2600f053e370-9b44-47e1-8f76-b22f1410a3776003f999534-b512-4847-ac36-c67c7dce0a0660012016000000-0002-7568-47561075600689d00d1-9ec3-49c2-b818-2f04e0d01a2060011206000000-0001-8587-458010636004f7e99d3-09d0-4213-9f4b-f236840661dc600d1d5a872-15eb-4364-ab9b-67360f3b74ca6004fde8c9c-d541-4ee0-bb88-60b59f906892600d180b3e7-5c80-430e-97b8-2d23c70cd5ca600Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud2025-04-02T17:31:30Z2025-04-02T17:31:30Z2025-03-18de Bilbao, N. V., Giebelhaus, R. T., Dias, R. P., Ferreira, M. E., Martínez, M., Velasco Carneros, L., Nam, S. L., de la Mata, A. P., Maréchal, J. D., Adou, A. I., Yaluff, G., Serna, E., Sylvestre, M., Torres, S., Schinini, A., Galeano, R., Fournet, A., Harynuk, J. J., & Cebrián Torrejón, G. (2025). Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking. Plants, 14(6), Artículo 954. https://doi.org/10.3390/plants14060954https://doi.org/10.3390/plants14060954http://hdl.handle.net/20.500.14066/455210.3390/plants140609542223-7747Correspondence: nverabilbao@gmail.com (N.V.d.B.); alain.fournet@orange.fr (A.F.)This article belongs to the Section Phytochemistry.This publication reports the controlled cultivation of Zanthoxylum chiloperone var. angustifolium Engl. (Rutaceae) in several growth substrates under controlled greenhouse conditions. This plant is well-known for its anti-Chagas (trypanocidal) activity, related to the presence of several β-carboline alkaloids. The metabolomic study of Z. chiloperone seedlings over two years of growth (2018–2020) was performed using comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS). The canthin-6-one alkaloids, canthin-6-one and 5-methoxy-canthin-6-one, were putatively identified in Z. chiloperone extracts. Finally, in vitro and in silico studies of trypanocidal activity were performed, suggesting that canthin-6-one alkaloids could interact with the main pharmacological targets against Trypanosoma cruzi, cruzain protease, dihydroorotate dehydrogenase, lanosterol 14-alpha-demethylase, farnesyl diphosphate, and squalene synthases.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrolloengMultidisciplinary Digital Publishing InstituteAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations6. Producción y tecnología industrialCanthin-6-onesChagas diseaseMedicinal plantsNatural productsPlant metabolomicsRutaceaeExploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand dockinginfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion6Plants14-INV-15114Vera de Bilbao, NinfaGiebelhaus, Ryland T.Dias, Ryan P.Ferreira de Paredes, María ElenaMartínez, MiguelVelasco Carneros, LoreaNam, Seo Linde la Mata, Aidee PaulinaMaréchal, Jean-DidierAdou, Ahissan InnocentYaluff Miranda, Gloria CristinaSerna, María ElvaSylvestre, MurielTorres de Ortiz, Agueda SusanaSchinini Vega, Alicia PetronaGaleano, RicardoFournet, AlainHarynuk, James J.Cebrian Torrejon, GerardoORIGINALExploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking.pdfExploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking.pdfArtículo científicoapplication/pdf1076868http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4552/1/Exploring%20the%20anti-Chagas%20activity%20of%20Zanthoxylum%20chiloperone%e2%80%99s%20seedlings%20through%20metabolomics%20and%20protein-ligand%20docking.pdfea4d77123c711e19e2fef8994dd1185bMD51Material suplementario.pdfMaterial suplementario.pdfMaterial suplementarioapplication/pdf597410http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4552/4/Material%20suplementario.pdf37132277c7f6e8dbbbeb7c3001b15ee9MD54CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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spellingShingle Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
Vera de Bilbao, Ninfa
6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations
6. Producción y tecnología industrial
Canthin-6-ones
Chagas disease
Medicinal plants
Natural products
Plant metabolomics
Rutaceae
status_str publishedVersion
title Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
title_full Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
title_fullStr Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
title_full_unstemmed Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
title_short Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
title_sort Exploring the anti-Chagas activity of Zanthoxylum chiloperone’s seedlings through metabolomics and protein-ligand docking
topic 6.16. Manufacture of basic pharmaceutical products and pharmaceutical preparations
6. Producción y tecnología industrial
Canthin-6-ones
Chagas disease
Medicinal plants
Natural products
Plant metabolomics
Rutaceae
url https://doi.org/10.3390/plants14060954
http://hdl.handle.net/20.500.14066/4552