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Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay

Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay. E-mail addresses: colepuerto@hotmail.com, medicinatropical@iics.una.py. (F. del Puerto).

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Main Author: del Puerto Rodas, Ramona Florencia (author)
Other Authors: Ozorio, Mónica (author), Trinidad, Beatriz (author), Martínez, Nidia (author), Torales, Martha Rossana (author), Franco, Luciano (author), Ferreira, Luis (author), Vera, Ninfa Isabel (author)
Format: article
Language:English
Published: 2020
Subjects:
7. Salud
Malaria
PCR
Paraguay
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
Online Access:http://hdl.handle.net/20.500.14066/2979
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author del Puerto Rodas, Ramona Florencia
author2 Ozorio, Mónica
Trinidad, Beatriz
Martínez, Nidia
Torales, Martha Rossana
Franco, Luciano
Ferreira, Luis
Vera, Ninfa Isabel
author2_role author
author
author
author
author
author
author
author_browse Ferreira, Luis
Franco, Luciano
Martínez, Nidia
Ozorio, Mónica
Torales, Martha Rossana
Trinidad, Beatriz
Vera, Ninfa Isabel
del Puerto Rodas, Ramona Florencia
author_facet del Puerto Rodas, Ramona Florencia
Ozorio, Mónica
Trinidad, Beatriz
Martínez, Nidia
Torales, Martha Rossana
Franco, Luciano
Ferreira, Luis
Vera, Ninfa Isabel
author_role author
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dc.contributor.other.es.fl_str_mv Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud
dc.creator.none.fl_str_mv del Puerto Rodas, Ramona Florencia
Ozorio, Mónica
Trinidad, Beatriz
Martínez, Nidia
Torales, Martha Rossana
Franco, Luciano
Ferreira, Luis
Vera, Ninfa Isabel
dc.date.accessioned.none.fl_str_mv 2022-04-21T01:59:38Z
dc.date.available.none.fl_str_mv 2022-04-21T01:59:38Z
dc.date.issued.none.fl_str_mv 2020-08-15
dc.format.extent.es.fl_str_mv 7 páginas
dc.identifier.doi.es.fl_str_mv 10.1016/j.parepi.2020.e00174
dc.identifier.essn.none.fl_str_mv 2405-6731
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.14066/2979
dc.language.iso.es.fl_str_mv eng
dc.publisher.none.fl_str_mv Elsevier
dc.relation.projectCONACYT.es.fl_str_mv 14-INV-463
dc.rights.*.fl_str_mv Atribución 4.0 Internacional
dc.rights.accessRights.es.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.copyright.none.fl_str_mv © 2020 The Author(s). Published by Elsevier Ltd on behalf of World Federation of Parasitologists
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.subject.classification.es.fl_str_mv 7. Salud
dc.subject.ocde.es.fl_str_mv 7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
dc.subject.other.es.fl_str_mv Malaria
PCR
Paraguay
dc.title.es.fl_str_mv Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
dc.type.es.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay. E-mail addresses: colepuerto@hotmail.com, medicinatropical@iics.una.py. (F. del Puerto).
eu_rights_str_mv openAccess
format article
id CONACYT_e5d4acd1c2b20ef8f65f331b6f6e12e1
identifier_str_mv 10.1016/j.parepi.2020.e00174
2405-6731
language eng
network_acronym_str CONACYT
network_name_str Repositorio Institucional CONACYT
oai_identifier_str oai:repositorio.conacyt.gov.py:20.500.14066/2979
publishDate 2020
publishDateSort 2020
publisher.none.fl_str_mv Elsevier
repository.mail.fl_str_mv repositorio.institucional@conacyt.gov.py
repository.name.fl_str_mv Repositorio Institucional CONACYT
repository_id_str
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
© 2020 The Author(s). Published by Elsevier Ltd on behalf of World Federation of Parasitologists
spelling 34060085060011256001e16394f-172b-486c-b0c8-7111fd55e4f06001117600f0f783b7-86cb-4107-a173-57af408c9cd5600949e569c-7e10-46ab-b63b-0c437a7f2b6c6001158600Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud2022-04-21T01:59:38Z2022-04-21T01:59:38Z2020-08-15http://hdl.handle.net/20.500.14066/297910.1016/j.parepi.2020.e001742405-6731Corresponding author at: Department of Tropical Medicine, Research Institute in Health Science (IICS-UNA), National University of Asunción, Campus UNA, San Lorenzo, Paraguay. E-mail addresses: colepuerto@hotmail.com, medicinatropical@iics.una.py. (F. del Puerto).In Paraguay, no cases of Malaria have been recorded since 2011. Microscopy and the SnM-PCR technique were implemented to detect and characterize Plasmodium spp. both in mosquitoes and in humans residing in historically endemic areas of Paraguay, to evaluate the possibility of finding asymptomatic cases and/or Plasmodium parasites circulating in anophelines. Between 2013 and 2015, 361 human blood samples were collected on filter paper, and between 2016 and 2017, 938 female Anopheles mosquitoes were captured in 15 Paraguayan localities. All the diagnostic techniques showed negative results. We observed no asymptomatic case or Plasmodium circulating in vectors.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Proyectos de investigación y desarrollo7 páginasengElsevierAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess© 2020 The Author(s). Published by Elsevier Ltd on behalf of World Federation of Parasitologists7. SaludMalariaPCRParaguay7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisiblesDetection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguayinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionParasite Epidemiology and Control14-INV-46311del Puerto Rodas, Ramona FlorenciaOzorio, MónicaTrinidad, BeatrizMartínez, NidiaTorales, Martha RossanaFranco, LucianoFerreira, LuisVera, Ninfa IsabelORIGINALDetection and characterization of Plasmodium spp.Detection and characterization of Plasmodium spp.Artículo científicoapplication/pdf547950http://repositorio.conacyt.gov.py/bitstream/20.500.14066/2979/1/Detection%20and%20characterization%20of%20Plasmodium%20spp.a69ec73636fb44464b6ff460b9e7b1e3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/2979/2/license.txt858b22fda432bd774e469302988c1974MD52TEXT14-inv-463art2.pdf.txt14-inv-463art2.pdf.txtExtracted texttext/plain26540http://repositorio.conacyt.gov.py/bitstream/20.500.14066/2979/3/14-inv-463art2.pdf.txt5b0c05f0d4a838704b80eb4ab1b0a32fMD53Detection and characterization of Plasmodium spp..txtDetection and characterization of Plasmodium spp..txtExtracted texttext/plain26540http://repositorio.conacyt.gov.py/bitstream/20.500.14066/2979/5/Detection%20and%20characterization%20of%20Plasmodium%20spp..txt5b0c05f0d4a838704b80eb4ab1b0a32fMD55THUMBNAILDetection and characterization of Plasmodium spp..jpgDetection and characterization of Plasmodium spp..jpgPortadaimage/jpeg922051http://repositorio.conacyt.gov.py/bitstream/20.500.14066/2979/4/Detection%20and%20characterization%20of%20Plasmodium%20spp..jpgbc176c89b56256b88ea70f7a674af153MD5420.500.14066/2979oai:repositorio.conacyt.gov.py:20.500.14066/29792026-02-12 19:30:26.548Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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
spellingShingle Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
del Puerto Rodas, Ramona Florencia
7. Salud
Malaria
PCR
Paraguay
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
status_str publishedVersion
title Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
title_full Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
title_fullStr Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
title_full_unstemmed Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
title_short Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
title_sort Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR both in mosquito vectors and in humans residing in historically endemic areas of Paraguay
topic 7. Salud
Malaria
PCR
Paraguay
7.3. Prevención, vigilancia y control de enfermedades transmisibles y no transmisibles
url http://hdl.handle.net/20.500.14066/2979

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