A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions
Saccharomyces cerevisiae mutants lacking both isoforms of the main plasma membrane potassium transporter display impaired potassium transport and defective growth at limiting concentrations of the cation. Moreover, they are hyperpolarized and have a lower intracellular pH than wild-type. In order to...
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| Outros autores: | , , , |
| Formato: | article |
| Idioma: | Lingua castelá |
| Publicado: |
2015
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| Acceso en liña: | http://repo.ulatina.edu.pa/handle/123456789/12 |
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| _version_ | 1869652473263685632 |
|---|---|
| author | Gelis, Samuel |
| author2 | Raquel, González-Fernández Rito, Herrera Jesús, Jorrín José, Ramos |
| author2_role | author author author author |
| author_browse | Gelis, Samuel Jesús, Jorrín José, Ramos Raquel, González-Fernández Rito, Herrera |
| author_facet | Gelis, Samuel Raquel, González-Fernández Rito, Herrera Jesús, Jorrín José, Ramos |
| author_role | author |
| collection | Repositorio de la Universidad Latina de Panamá |
| dc.creator.none.fl_str_mv | Gelis, Samuel Raquel, González-Fernández Rito, Herrera Jesús, Jorrín José, Ramos |
| dc.date.none.fl_str_mv | 2015-06-01 2020-02-14T21:03:58Z 2020-02-14T21:03:58Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | 1350-0872 1465-2080 10.1099/mic.0.000078 http://repo.ulatina.edu.pa/handle/123456789/12 |
| dc.language.none.fl_str_mv | spa |
| dc.publisher.none.fl_str_mv | MICROBIOLOGY |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositorio de la Universidad Latina de Panamá instname:Universidad Latina de Panamá instacron:ULATINA |
| dc.title.none.fl_str_mv | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Saccharomyces cerevisiae mutants lacking both isoforms of the main plasma membrane potassium transporter display impaired potassium transport and defective growth at limiting concentrations of the cation. Moreover, they are hyperpolarized and have a lower intracellular pH than wild-type. In order to unravel global physiological processes altered in trk1,2 mutants, we have established conditions at which both wild-type and mutants can grow at different rates. Using a combination of physiological, biochemical and proteomic approaches, we show that during growth at suboptimal potassium concentrations, double trk1,2 mutants accumulate less potassium and reach lower yields. In contrast, the mutants maintain increased viability in the stationary phase and retain more potassium. Moreover, the mutants show increased expression of stress-related proteins such as catalase T, thioredoxin peroxidase or hexokinase 2, suggesting that they are better adapted to the additional stress factors associated with entry into stationary growth phase. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | lrtest_b0507b5042a12c6c367e1d76a66e2f01 |
| identifier_str_mv | 1350-0872 1465-2080 10.1099/mic.0.000078 |
| instacron_str | ULATINA |
| institution | ULATINA |
| instname_str | Universidad Latina de Panamá |
| language | spa |
| network_acronym_str | lrtest |
| network_name_str | lr |
| oai_identifier_str | oai:repo.ulatina.edu.pa:123456789/12 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher.none.fl_str_mv | MICROBIOLOGY |
| reponame_str | Repositorio de la Universidad Latina de Panamá |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| spelling | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditionsGelis, SamuelRaquel, González-FernándezRito, HerreraJesús, JorrínJosé, RamosSaccharomyces cerevisiae mutants lacking both isoforms of the main plasma membrane potassium transporter display impaired potassium transport and defective growth at limiting concentrations of the cation. Moreover, they are hyperpolarized and have a lower intracellular pH than wild-type. In order to unravel global physiological processes altered in trk1,2 mutants, we have established conditions at which both wild-type and mutants can grow at different rates. Using a combination of physiological, biochemical and proteomic approaches, we show that during growth at suboptimal potassium concentrations, double trk1,2 mutants accumulate less potassium and reach lower yields. In contrast, the mutants maintain increased viability in the stationary phase and retain more potassium. Moreover, the mutants show increased expression of stress-related proteins such as catalase T, thioredoxin peroxidase or hexokinase 2, suggesting that they are better adapted to the additional stress factors associated with entry into stationary growth phase.Saccharomyces cerevisiae mutants lacking both isoforms of the main plasma membrane potassium transporter display impaired potassium transport and defective growth at limiting concentrations of the cation. Moreover, they are hyperpolarized and have a lower intracellular pH than wild-type. In order to unravel global physiological processes altered in trk1,2 mutants, we have established conditions at which both wild-type and mutants can grow at different rates. Using a combination of physiological, biochemical and proteomic approaches, we show that during growth at suboptimal potassium concentrations, double trk1,2 mutants accumulate less potassium and reach lower yields. In contrast, the mutants maintain increased viability in the stationary phase and retain more potassium. Moreover, the mutants show increased expression of stress-related proteins such as catalase T, thioredoxin peroxidase or hexokinase 2, suggesting that they are better adapted to the additional stress factors associated with entry into stationary growth phase.MICROBIOLOGY2020-02-14T21:03:58Z2020-02-14T21:03:58Z2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1350-08721465-208010.1099/mic.0.000078http://repo.ulatina.edu.pa/handle/123456789/12spainfo:eu-repo/semantics/openAccessreponame:Repositorio de la Universidad Latina de Panamáinstname:Universidad Latina de Panamáinstacron:ULATINAoai:repo.ulatina.edu.pa:123456789/122020-03-02T22:32:56Z |
| spellingShingle | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions Gelis, Samuel |
| status_str | publishedVersion |
| title | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| title_full | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| title_fullStr | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| title_full_unstemmed | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| title_short | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| title_sort | A physiological, biochemical and proteomic characterization of Saccharomyces cerevisiae trk1,2 transport mutants grown under limiting potassium conditions |
| url | http://repo.ulatina.edu.pa/handle/123456789/12 |