Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs
Systematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit–variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion...
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| Other Authors: | , , , , , |
| Format: | article |
| Language: | English |
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2019
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| Online Access: | http://repositorio-indicasat.org.pa/handle/123456789/6 |
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| _version_ | 1869652476775366656 |
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| author | Domínguez, Juan |
| author2 | Acosta, Fermín Pérez-Lago, Laura Sambrano, Dilcia Goodridge, Amador Batista, Victoria De La Guardia, Carolina |
| author2_role | author author author author author author |
| author_browse | Acosta, Fermín Batista, Victoria De La Guardia, Carolina Domínguez, Juan Goodridge, Amador Pérez-Lago, Laura Sambrano, Dilcia |
| author_facet | Domínguez, Juan Acosta, Fermín Pérez-Lago, Laura Sambrano, Dilcia Goodridge, Amador Batista, Victoria De La Guardia, Carolina |
| author_role | author |
| collection | Repositorio INDICASAT |
| dc.creator.none.fl_str_mv | Domínguez, Juan Acosta, Fermín Pérez-Lago, Laura Sambrano, Dilcia Goodridge, Amador Batista, Victoria De La Guardia, Carolina |
| dc.date.none.fl_str_mv | 2019-03-03 2020-02-09T19:12:29Z 2020-02-09T19:12:29Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | http://repositorio-indicasat.org.pa/handle/123456789/6 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Emerging Infectious Diseases |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.source.none.fl_str_mv | reponame:Repositorio INDICASAT instname:Instituto de Investigaciones Científicas y Servicios de Alta Tecnología instacron:INDICASAT |
| dc.subject.none.fl_str_mv | Tuberculosis model, surveillance bacteria tuberculosis and other mycobacteria epidemiology transmission whole-genome sequencing strain-specific PCR |
| dc.title.none.fl_str_mv | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Systematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit–variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A–F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism–based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain–specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A–C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain–specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programs |
| eu_rights_str_mv | openAccess |
| format | article |
| id | lrtest_e76c8c668c05eccdb29f739e241ef1b1 |
| instacron_str | INDICASAT |
| institution | INDICASAT |
| instname_str | Instituto de Investigaciones Científicas y Servicios de Alta Tecnología |
| language | eng |
| network_acronym_str | lrtest |
| network_name_str | lr |
| oai_identifier_str | oai:repositorio-indicasat.org.pa:123456789/6 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Emerging Infectious Diseases |
| reponame_str | Repositorio INDICASAT |
| repository.mail.fl_str_mv | mail@mail.com |
| repository.name.fl_str_mv |
-
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| repository_id_str | |
| rights_invalid_str_mv | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| spelling | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology ProgramsDomínguez, JuanAcosta, FermínPérez-Lago, LauraSambrano, DilciaGoodridge, AmadorBatista, VictoriaDe La Guardia, CarolinaTuberculosismodel, surveillancebacteriatuberculosis and other mycobacteriaepidemiologytransmissionwhole-genome sequencingstrain-specific PCRSystematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit–variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A–F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism–based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain–specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A–C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain–specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programsSystematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit–variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A–F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism–based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain–specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A–C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain–specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programsEmerging Infectious Diseases2020-02-09T19:12:29Z2020-02-09T19:12:29Z2019-03-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://repositorio-indicasat.org.pa/handle/123456789/6enginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/reponame:Repositorio INDICASATinstname:Instituto de Investigaciones Científicas y Servicios de Alta Tecnologíainstacron:INDICASAT2020-05-27T10:06:44Zmail@mail.com - |
| spellingShingle | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs Domínguez, Juan Tuberculosis model, surveillance bacteria tuberculosis and other mycobacteria epidemiology transmission whole-genome sequencing strain-specific PCR |
| status_str | publishedVersion |
| title | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| title_full | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| title_fullStr | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| title_full_unstemmed | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| title_short | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| title_sort | Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs |
| topic | Tuberculosis model, surveillance bacteria tuberculosis and other mycobacteria epidemiology transmission whole-genome sequencing strain-specific PCR |
| url | http://repositorio-indicasat.org.pa/handle/123456789/6 |