প্রচ্ছদ

Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules

For correspondence: María José Fernández Nestosa, mjfernandez@pol.una.py

সংরক্ষণ করুন:
প্রধান লেখক: Cebrián, Jorge (author)
অন্যান্য লেখক: Martínez, Víctor (author), Hernández, Pablo (author), Krimer, Dora B. (author), Martínez Robles, María Luisa (author), Schvartzman, Jorge B. (author), Fernández de Nestosa, María José (author)
বিন্যাস: article
ভাষা:ইংরেজি
প্রকাশিত: 2024
বিষয়গুলি:
2D gels
Catenation
DNA topology
Knots
Supercoiling
Topoisomerases
অনলাইন ব্যবহার করুন:http://hdl.handle.net/20.500.14066/4419
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author Cebrián, Jorge
author2 Martínez, Víctor
Hernández, Pablo
Krimer, Dora B.
Martínez Robles, María Luisa
Schvartzman, Jorge B.
Fernández de Nestosa, María José
author2_role author
author
author
author
author
author
author_browse Cebrián, Jorge
Fernández de Nestosa, María José
Hernández, Pablo
Krimer, Dora B.
Martínez Robles, María Luisa
Martínez, Víctor
Schvartzman, Jorge B.
author_facet Cebrián, Jorge
Martínez, Víctor
Hernández, Pablo
Krimer, Dora B.
Martínez Robles, María Luisa
Schvartzman, Jorge B.
Fernández de Nestosa, María José
author_role author
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dc.creator.none.fl_str_mv Cebrián, Jorge
Martínez, Víctor
Hernández, Pablo
Krimer, Dora B.
Martínez Robles, María Luisa
Schvartzman, Jorge B.
Fernández de Nestosa, María José
dc.date.accessioned.none.fl_str_mv 2024-07-04T13:51:15Z
dc.date.available.none.fl_str_mv 2024-07-04T13:51:15Z
dc.date.issued.none.fl_str_mv 2024-05-05
dc.identifier.citation.en.fl_str_mv Cebrián, J., Martínez, V., Hernández, P., Krimer, D. B., Martínez-Robles, M. L., Schvartzman, J. B. and Fernández-Nestosa, M. J. (2024). Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules. Bio-protocol 14(9): e4983. DOI: 10.21769/BioProtoc.4983.
dc.identifier.doi.es.fl_str_mv 10.21769/BioProtoc.4983
dc.identifier.issn.es.fl_str_mv 2331-8325
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.14066/4419
dc.language.iso.es.fl_str_mv eng
dc.publisher.es.fl_str_mv Bio-protocol LLC
dc.rights.*.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.accessRights.es.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.copyright.es.fl_str_mv © 2024 The Authors; exclusive licensee Bio-protocol LLC.
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.subject.other.es.fl_str_mv 2D gels
Catenation
DNA topology
Knots
Supercoiling
Topoisomerases
dc.title.es.fl_str_mv Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
dc.type.es.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description For correspondence: María José Fernández Nestosa, mjfernandez@pol.una.py
eu_rights_str_mv openAccess
format article
id CONACYT_0b585bd870d3b78a86fd6d9ddb37f484
identifier_str_mv Cebrián, J., Martínez, V., Hernández, P., Krimer, D. B., Martínez-Robles, M. L., Schvartzman, J. B. and Fernández-Nestosa, M. J. (2024). Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules. Bio-protocol 14(9): e4983. DOI: 10.21769/BioProtoc.4983.
2331-8325
10.21769/BioProtoc.4983
language eng
network_acronym_str CONACYT
network_name_str Repositorio Institucional CONACYT
oai_identifier_str oai:repositorio.conacyt.gov.py:20.500.14066/4419
publishDate 2024
publishDateSort 2024
repository.mail.fl_str_mv repositorio.institucional@conacyt.gov.py
repository.name.fl_str_mv Repositorio Institucional CONACYT
repository_id_str
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
© 2024 The Authors; exclusive licensee Bio-protocol LLC.
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The possibility to use E. coli strains with different genetic backgrounds in combination with nicking enzymes and different concentrations of norfloxacin improves the resolution of 2D gels to study the electrophoretic behavior of three different families of DNA topoisomers: supercoiled DNA molecules, post-replicative catenanes, and knotted DNA molecules. Here, we describe the materials and procedures required to optimize their separation by 2D gels. Understanding the differences in their electrophoretic behavior can help explain some important physical characteristics of these different types of DNA topoisomers.Consejo Nacional de Ciencia y TecnologíaPrograma Paraguayo para el Desarrollo de la Ciencia y Tecnología. Programa Nacional de Incentivo a los InvestigadoresengBio-protocol LLCAtribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccess© 2024 The Authors; exclusive licensee Bio-protocol LLC.2D gelsCatenationDNA topologyKnotsSupercoilingTopoisomerasesElectrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA moleculesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9Bio-protocol14Cebrián, JorgeMartínez, VíctorHernández, PabloKrimer, Dora B.Martínez Robles, María LuisaSchvartzman, Jorge B.Fernández de Nestosa, María JoséORIGINALElectrophoretic mobility assay to separate supercoiled.pdfElectrophoretic mobility assay to separate supercoiled.pdfArtículo científicoapplication/pdf1605281http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4419/1/Electrophoretic%20mobility%20assay%20to%20separate%20supercoiled.pdfe9171806f7978e81b1898ba619731f9aMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4419/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4419/3/license.txt858b22fda432bd774e469302988c1974MD53THUMBNAILElectrophoretic mobility assay to separate supercoiled.jpgElectrophoretic mobility assay to separate supercoiled.jpgPortadaimage/jpeg607499http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4419/4/Electrophoretic%20mobility%20assay%20to%20separate%20supercoiled.jpg3f3607ba97f5f7201542b4029e4ac218MD54TEXTElectrophoretic mobility assay to separate supercoiled.pdf.txtElectrophoretic mobility assay to separate supercoiled.pdf.txtExtracted texttext/plain48530http://repositorio.conacyt.gov.py/bitstream/20.500.14066/4419/5/Electrophoretic%20mobility%20assay%20to%20separate%20supercoiled.pdf.txt1e9c85f3d9c577eacdfcae9b84c1ec4eMD5520.500.14066/4419oai:repositorio.conacyt.gov.py:20.500.14066/44192026-02-12 19:30:36.095Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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
spellingShingle Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
Cebrián, Jorge
2D gels
Catenation
DNA topology
Knots
Supercoiling
Topoisomerases
status_str publishedVersion
title Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_full Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_fullStr Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_full_unstemmed Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_short Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_sort Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
topic 2D gels
Catenation
DNA topology
Knots
Supercoiling
Topoisomerases
url http://hdl.handle.net/20.500.14066/4419

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