Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo
The topology of DNA duplexes changes during replication and also after deproteinization in vitro. Here we describe these changes and then discuss for the first time how the distribution of superhelical stress affects the DNA topology of replication intermediates, taking into account the progression...
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| Sprache: | Englisch |
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| Online-Zugang: | http://hdl.handle.net/20.500.14066/3833 https://doi.org/10.1002/bies.202000309 |
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| _version_ | 1870612064803749888 |
|---|---|
| author | Schvartzman, Jorge Bernardo |
| author2 | Martínez, Víctor Hernández, Pablo Krimer, Dora B. Fernández de Nestosa, María José |
| author2_role | author author author author |
| author_browse | Fernández de Nestosa, María José Hernández, Pablo Krimer, Dora B. Martínez, Víctor Schvartzman, Jorge Bernardo |
| author_facet | Schvartzman, Jorge Bernardo Martínez, Víctor Hernández, Pablo Krimer, Dora B. Fernández de Nestosa, María José |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3833/1/PINV15-573art1.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3833/2/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3833/3/PINV15-573art1.pdf.txt |
| dc.contributor.other.es.fl_str_mv | Universidad Nacional de Asunción - Facultad Politécnica |
| dc.creator.none.fl_str_mv | Schvartzman, Jorge Bernardo Martínez, Víctor Hernández, Pablo Krimer, Dora B. Fernández de Nestosa, María José |
| dc.date.accessioned.none.fl_str_mv | 2022-05-02T18:11:58Z |
| dc.date.available.none.fl_str_mv | 2022-05-02T18:11:58Z |
| dc.date.issued.none.fl_str_mv | 2021 |
| dc.identifier.doi.es.fl_str_mv | https://doi.org/10.1002/bies.202000309 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/3833 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.relation.projectCONACYT.es.fl_str_mv | PINV15-573 |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.copyright.es.fl_str_mv | © 2021 Wiley Periodicals LLC |
| dc.subject.classification.es.fl_str_mv | 7. Salud |
| dc.subject.other.es.fl_str_mv | CATENATION DNA CHIRALITY DNA TOPOLOGY PRE-CATENATION REPLICATION SUPERCOILING |
| dc.title.es.fl_str_mv | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | The topology of DNA duplexes changes during replication and also after deproteinization in vitro. Here we describe these changes and then discuss for the first time how the distribution of superhelical stress affects the DNA topology of replication intermediates, taking into account the progression of replication forks. The high processivity of Topo IV to relax the left-handed (+) supercoiling that transiently accumulates ahead of the forks is not essential, since DNA gyrase and swiveling of the forks cooperate with Topo IV to accomplish this task in vivo. We conclude that despite Topo IV has a lower processivity to unlink the right-handed (+) crossings of pre-catenanes and fully replicated catenanes, this is indeed its main role in vivo. This would explain why in the absence of Topo IV replication goes-on, but fully replicated sister duplexes remain heavily catenated. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_0aeffc523391a88e8fb455322a5a611e |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/3833 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | © 2021 Wiley Periodicals LLC |
| spelling | 1065600ae34b808-b1a2-47fe-9cdf-c0940be361fc600644c6599-893c-4718-9a69-f27695fa83916001a615720-7aa0-4b4c-b423-547a688589cf600411600Universidad Nacional de Asunción - Facultad Politécnica2022-05-02T18:11:58Z2022-05-02T18:11:58Z2021http://hdl.handle.net/20.500.14066/3833https://doi.org/10.1002/bies.202000309The topology of DNA duplexes changes during replication and also after deproteinization in vitro. Here we describe these changes and then discuss for the first time how the distribution of superhelical stress affects the DNA topology of replication intermediates, taking into account the progression of replication forks. The high processivity of Topo IV to relax the left-handed (+) supercoiling that transiently accumulates ahead of the forks is not essential, since DNA gyrase and swiveling of the forks cooperate with Topo IV to accomplish this task in vivo. We conclude that despite Topo IV has a lower processivity to unlink the right-handed (+) crossings of pre-catenanes and fully replicated catenanes, this is indeed its main role in vivo. This would explain why in the absence of Topo IV replication goes-on, but fully replicated sister duplexes remain heavily catenated.Consejo Nacional de Ciencia y TecnologíaPROCIENCIAeng7. SaludCATENATIONDNA CHIRALITYDNA TOPOLOGYPRE-CATENATIONREPLICATIONSUPERCOILINGChanges in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBioEssays17PINV15-573info:eu-repo/semantics/openAccess© 2021 Wiley Periodicals LLCSchvartzman, Jorge BernardoMartínez, VíctorHernández, PabloKrimer, Dora B.Fernández de Nestosa, María JoséORIGINALPINV15-573art1.pdfPINV15-573art1.pdfapplication/pdf3035389http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3833/1/PINV15-573art1.pdf8a378d1946edd49c022a7d4c593e9bebMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3833/2/license.txt858b22fda432bd774e469302988c1974MD52TEXTPINV15-573art1.pdf.txtPINV15-573art1.pdf.txtExtracted texttext/plain34908http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3833/3/PINV15-573art1.pdf.txtbcaca24e4089b3ae947dbdcbf7cc9f78MD5320.500.14066/3833oai:repositorio.conacyt.gov.py:20.500.14066/38332026-02-12 19:30:30.724Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo Schvartzman, Jorge Bernardo 7. Salud CATENATION DNA CHIRALITY DNA TOPOLOGY PRE-CATENATION REPLICATION SUPERCOILING |
| status_str | publishedVersion |
| title | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| title_full | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| title_fullStr | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| title_full_unstemmed | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| title_short | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| title_sort | Changes in the topology of DNA replication intermediates: Important discrepancies between in vitro and in vivo |
| topic | 7. Salud CATENATION DNA CHIRALITY DNA TOPOLOGY PRE-CATENATION REPLICATION SUPERCOILING |
| url | http://hdl.handle.net/20.500.14066/3833 https://doi.org/10.1002/bies.202000309 |