Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates
DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined. However, their ability to relax supercoiling and unlink the pre-c...
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| Médium: | article |
| Jazyk: | angličtina |
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2021
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| On-line přístup: | http://hdl.handle.net/20.500.14066/3839 |
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| _version_ | 1870885388477792256 |
|---|---|
| author | Cebrian, Jorge |
| author2 | Martínez, Víctor Hernández, Pablo Krimer, Dora Fernández Nestosa, María José Schvartzman, Jorge Bernardo |
| author2_role | author author author author author |
| author_browse | Cebrian, Jorge Fernández Nestosa, María José Hernández, Pablo Krimer, Dora Martínez, Víctor Schvartzman, Jorge Bernardo |
| author_facet | Cebrian, Jorge Martínez, Víctor Hernández, Pablo Krimer, Dora Fernández Nestosa, María José Schvartzman, Jorge Bernardo |
| author_role | author |
| bitstream.checksum.fl_str_mv | 104652e6c106da8d092ef7d41d5b8fd1 858b22fda432bd774e469302988c1974 7a1b64b5fc0a139fffd9479b2b584149 |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 MD5 |
| bitstream.url.fl_str_mv | http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3839/1/PINV15-573art2.pdf http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3839/2/license.txt http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3839/3/PINV15-573art2.pdf.txt |
| dc.contributor.other.es.fl_str_mv | Universidad Nacional de Asunción - Facultad Politécnica |
| dc.creator.none.fl_str_mv | Cebrian, Jorge Martínez, Víctor Hernández, Pablo Krimer, Dora Fernández Nestosa, María José Schvartzman, Jorge Bernardo |
| dc.date.accessioned.none.fl_str_mv | 2022-05-02T18:18:28Z |
| dc.date.available.none.fl_str_mv | 2022-05-02T18:18:28Z |
| dc.date.issued.none.fl_str_mv | 2021-11 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.14066/3839 |
| dc.language.iso.es.fl_str_mv | eng |
| dc.relation.projectCONACYT.es.fl_str_mv | PINV15-573 |
| dc.rights.accessRights.es.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.rights.copyright.es.fl_str_mv | © 2021 by the authors. |
| dc.subject.classification.es.fl_str_mv | 1303 I+D en relación con las Ciencias médicas |
| dc.subject.ocde.es.fl_str_mv | SISTEMAS DE INFORMACION |
| dc.subject.other.es.fl_str_mv | DNA TOPOLOGY REPLICATION SUPERCOILING PRE-CATENATION TWO-DIMENSIONAL AGAROSE GEL ELECTROPHORESIS |
| dc.title.es.fl_str_mv | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| dc.type.es.fl_str_mv | info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined. However, their ability to relax supercoiling and unlink the pre-catenanes of partially replicated molecules has received little attention. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | CONACYT_9e9ccbe3b82c570e420c4ed7d9c25f02 |
| language | eng |
| network_acronym_str | CONACYT |
| network_name_str | Repositorio Institucional CONACYT |
| oai_identifier_str | oai:repositorio.conacyt.gov.py:20.500.14066/3839 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| repository.mail.fl_str_mv | repositorio.institucional@conacyt.gov.py |
| repository.name.fl_str_mv | Repositorio Institucional CONACYT |
| repository_id_str | |
| rights_invalid_str_mv | © 2021 by the authors. |
| spelling | dd12e1ff-2904-474d-a0bb-68f910365705600ae34b808-b1a2-47fe-9cdf-c0940be361fc600644c6599-893c-4718-9a69-f27695fa8391600a0699453-d881-4d8a-ada8-538c5042aaaf600ff0b2cf4-b420-42f1-8131-d09740b9c30c6001065600Universidad Nacional de Asunción - Facultad Politécnica2022-05-02T18:18:28Z2022-05-02T18:18:28Z2021-11http://hdl.handle.net/20.500.14066/3839DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined. However, their ability to relax supercoiling and unlink the pre-catenanes of partially replicated molecules has received little attention.Consejo Nacional de Ciencia y TecnologíaPROCIENCIAeng1303 I+D en relación con las Ciencias médicasDNA TOPOLOGYREPLICATIONSUPERCOILINGPRE-CATENATIONTWO-DIMENSIONAL AGAROSE GEL ELECTROPHORESISSISTEMAS DE INFORMACIONTwo-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediatesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11Journal of BiologyPINV15-573info:eu-repo/semantics/openAccess© 2021 by the authors.10Cebrian, JorgeMartínez, VíctorHernández, PabloKrimer, DoraFernández Nestosa, María JoséSchvartzman, Jorge BernardoORIGINALPINV15-573art2.pdfPINV15-573art2.pdfapplication/pdf1834970http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3839/1/PINV15-573art2.pdf104652e6c106da8d092ef7d41d5b8fd1MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81698http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3839/2/license.txt858b22fda432bd774e469302988c1974MD52TEXTPINV15-573art2.pdf.txtPINV15-573art2.pdf.txtExtracted texttext/plain73162http://repositorio.conacyt.gov.py/bitstream/20.500.14066/3839/3/PINV15-573art2.pdf.txt7a1b64b5fc0a139fffd9479b2b584149MD5320.500.14066/3839oai:repositorio.conacyt.gov.py:20.500.14066/38392026-02-12 19:30:32.465Repositorio Institucional CONACYTrepositorio.institucional@conacyt.gov.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 |
| spellingShingle | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates Cebrian, Jorge 1303 I+D en relación con las Ciencias médicas DNA TOPOLOGY REPLICATION SUPERCOILING PRE-CATENATION TWO-DIMENSIONAL AGAROSE GEL ELECTROPHORESIS SISTEMAS DE INFORMACION |
| status_str | publishedVersion |
| title | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| title_full | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| title_fullStr | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| title_full_unstemmed | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| title_short | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| title_sort | Two-dimensional gel electrophoresis to study the activity of type iia topoisomerases on plasmid replication intermediates |
| topic | 1303 I+D en relación con las Ciencias médicas DNA TOPOLOGY REPLICATION SUPERCOILING PRE-CATENATION TWO-DIMENSIONAL AGAROSE GEL ELECTROPHORESIS SISTEMAS DE INFORMACION |
| url | http://hdl.handle.net/20.500.14066/3839 |